Morphological and RAPD analysis of poplar trees of anther culture origin

J. Kiss, M. Kondrák, O. Törjék, E. Kiss, G. Gyulai, K. Mázik-Tökei, L. Heszky

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Our objectives were to improve the rate of haploid plant regeneration through increasing the rate of callus initiation on the anthers and sustaining shoot regeneration frequency, and to analyze the field population of anther culture origin by morphological and molecular methods. Regarding the callus initiation, the most responsive clones were 'N-90' (59%) in P. nigra and 'D-29' (75%) in P. deltoides. The rate of shoot regeneration and number of shoots/calli ranged from 4%-79% and 1-9, respectively. From the 208 rooted plants 8 haploid, 179 diploid, 4 tetraploid and 17 aneuploid plants were found. In the field population, the haploid plants could be easily identified by their retarded development and morphological characteristics (size and shape of the leaves, strong branching, etc). Several diploid plants showed depressed developmental and morphological traits similar to the haploid ones. Three traits (growth rate, leaf blade length and shape of leaf base) of the 6 different morphological characteristics measured were in correlation with the ploidy level within the poplar field population. Six primers of the 48 primers tested were able to detect polymorphism among the field plants.

Original languageEnglish
Pages (from-to)213-221
Number of pages9
JournalEuphytica
Volume118
Issue number2
DOIs
Publication statusPublished - 2001

Fingerprint

anther culture
Haploidy
haploidy
Bony Callus
Regeneration
callus
Diploidy
shoots
diploidy
Population
Tetraploidy
Ploidies
aneuploidy
Aneuploidy
growth traits
ploidy
leaf blade
anthers
tetraploidy
branching

Keywords

  • Anther culture
  • Field trial
  • Morphological and molecular characterization
  • Populus
  • RAPD analysis

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Horticulture
  • Plant Science

Cite this

Morphological and RAPD analysis of poplar trees of anther culture origin. / Kiss, J.; Kondrák, M.; Törjék, O.; Kiss, E.; Gyulai, G.; Mázik-Tökei, K.; Heszky, L.

In: Euphytica, Vol. 118, No. 2, 2001, p. 213-221.

Research output: Contribution to journalArticle

Kiss, J. ; Kondrák, M. ; Törjék, O. ; Kiss, E. ; Gyulai, G. ; Mázik-Tökei, K. ; Heszky, L. / Morphological and RAPD analysis of poplar trees of anther culture origin. In: Euphytica. 2001 ; Vol. 118, No. 2. pp. 213-221.
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