Morphological and proliferative studies on ex vivo cultured human anterior lens epithelial cells - Relevance to capsular opacification

Sofija Andjelic, Kazimir Drašlar, Xhevat Lumi, Xiaohe Yan, Joachim Graw, Andrea Facskõ, Marko Hawlina, Goran Petrovski

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Purpose To determine the structural characteristics of lens epithelial cells (LECs) found on the anterior portion of the lens capsule and their pluripotency, proliferating and migrating potential when grown ex vivo with relevance to posterior capsular opacification (PCO) after cataract surgery. Methods The explants of anterior portion of the lens capsule consisting of monolayer of LECs were obtained from uneventful cataract surgery and were cultivated under adherent conditions. The size and shape of the outgrowing cells were recorded by scanning electron microscopy (SEM), while their migration and proliferation potential were followed using light microscopy. Positivity for proliferation (Ki-67)- and pluripotency (Sox2)-specific markers were tested by immunofluorescent staining. Results The proliferation and migration of anterior portion of the lens capsule's LECs filling up the denuded and reverse side regions of the lens capsule as well as their growth on glass culture surfaces could be followed by light microscopy and SEM, while the distribution of LECs and their morphology could be analysed in detail by SEM. The expression of Ki-67 and Sox2 in LECs growing adherently on human anterior portion of the lens capsule could also be detected. Conclusions Classic light microscopy and SEM can be used to show that human anterior portion of the lens capsule harbours LECs that can proliferate and migrate, suggesting their pluripotency or putative stem cell nature. Similarly, morphological techniques can be used to study PCO and the effect different drugs or physical treatments have against PCO development.

Original languageEnglish
Pages (from-to)e499-e506
JournalActa Ophthalmologica
Volume93
Issue number6
DOIs
Publication statusPublished - Sep 2015

Keywords

  • lens epithelial cells
  • pluripotency
  • posterior capsular opacification
  • proliferation
  • scanning electron microscopy

ASJC Scopus subject areas

  • Ophthalmology

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