Morphological and molecular analysis of common millet (P. miliaceum) cultivars compared to an aDNA sample from the 15th century (Hungary)

R. Lágler, G. Gyulai, M. Humphreys, Z. Szabó, L. Horváth, A. Bittsánszky, J. Kiss, L. Holly, L. Heszky

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Morphological characterization of 20 common millet (Panicum miliaceum L., 2n = 4x = 36) cultivars and landraces revealed four distinct clusters which were apparently consistent with the grain colors of black, black and brown, red, yellow, and white. Seed remains of medieval millet, recovered from a 15th century layer (King's Palace, Budapest, Hungary), showed reddish yellow grain color after rehydrating on tissue culture medium that was close to grain color of modern cultivar Omszkoje. aDNA of medieval commom millet was extracted successfully, analyzed and compared to modern common millets by ISSR, SSR, CAP and mtDNA. Analyses of fragments and sequences revealed polymorphism at seven ISSR loci (15 alleles) and at the 5S-18S rDNA locus of mtDNA. CAP analysis of the 5S-18S rDNA fragment revealed no SNPs in the restriction sites of six endonucleases TaqI, BsuRI, HinfI, MboI, AluI and RsaI. Sequence alignments of the restriction fragments RsaI also revealed consensus sequence in the medieval sample compared to a modern variety. An attempted phenotype reconstruction indicated that medieval common millet showed the closest morphological similarity to modern millet cultivar Omszkoje.

Original languageEnglish
Pages (from-to)77-85
Number of pages9
JournalEuphytica
Volume146
Issue number1-2
DOIs
Publication statusPublished - Nov 2005

Keywords

  • AncientDNA
  • CAP
  • ISSR
  • SNPs
  • mtDNA

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science
  • Horticulture

Fingerprint Dive into the research topics of 'Morphological and molecular analysis of common millet (P. miliaceum) cultivars compared to an aDNA sample from the 15th century (Hungary)'. Together they form a unique fingerprint.

  • Cite this