Morphological and mitochondrial DNA characters for identification and phylogenetic analysis of the myiasis-causing flesh fly Wohlfahrtia magnifica and its relatives, with a description of Wohlfahrtia monegrosensis sp. n. Wyatt & Hall

M. J R Hall, Z. J O Adams, N. P. Wyatt, J. M. Testa, W. Edge, M. Nikolausz, R. Farkas, P. D. Ready

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Wohlfahrtia magnifica (Schiner) (Diptera: Sarcophagidae) is a major cause of traumatic myiasis in livestock in Central and Eastern Europe and in countries bordering the Mediterranean. The present study explored the utility of external body characters, genitalia characters and mitochondrial DNA characters for identification of this and related species in the subfamily Paramacronychiinae. Sequence analyses of the 3′ terminal 273 bp of the mitochondrial cytochrome b gene revealed two lineages of W. magnifica, one from Spain and France and the other from the rest of Eurasia, differing by only two base pairs. Phylogenetic analysis of cytochrome b showed that W. magnifica and Wohlfahrtia vigil Walker were sister species; this conclusion was not contradicted by a phylogenetic analysis of the morphological characters. Based on cytochrome b, the genetic distance between specimens of W. vigil from Europe and North America was sufficiently large to justify the recognition of more than one species. A new species, Wohlfahrtia monegrosensis, from northern Spain, was described, based on morphology and cytochrome b. A unique combination of external body characters of males or females were diagnostic for W. magnifica, the W. vigil group and Wohlfahrtia bella, but only the genitalia characters were diagnostic for all nine species studied.

Original languageEnglish
Pages (from-to)59-71
Number of pages13
JournalMedical and Veterinary Entomology
Volume23
Issue numberSUPPL. 1
DOIs
Publication statusPublished - Jun 2009

Fingerprint

Wohlfahrtia
Wohlfahrtia magnifica
Sarcophagidae
Myiasis
myiasis
Cytochromes b
Mitochondrial DNA
cytochrome b
mitochondrial DNA
cytochrome
phylogenetics
Genitalia
phylogeny
Spain
genitalia
Eastern Europe
Eastern European region
Eurasia
Livestock
North America

Keywords

  • Cytochrome b
  • Mitochondrial DNA
  • Morphology
  • Sarcophagidae
  • Wohlfahrtia magnifica
  • Wohlfahrtia monegrosensis

ASJC Scopus subject areas

  • Insect Science
  • veterinary(all)
  • Ecology, Evolution, Behavior and Systematics
  • Parasitology

Cite this

Morphological and mitochondrial DNA characters for identification and phylogenetic analysis of the myiasis-causing flesh fly Wohlfahrtia magnifica and its relatives, with a description of Wohlfahrtia monegrosensis sp. n. Wyatt & Hall. / Hall, M. J R; Adams, Z. J O; Wyatt, N. P.; Testa, J. M.; Edge, W.; Nikolausz, M.; Farkas, R.; Ready, P. D.

In: Medical and Veterinary Entomology, Vol. 23, No. SUPPL. 1, 06.2009, p. 59-71.

Research output: Contribution to journalArticle

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