Morphological and histochemical changes in intercellular junctional complexes in epithelial cells of mouse small intestine upon X-irradiation

Changes of ruthenium red permeability and calcium content

Z. Somosy, J. Kovács, L. Siklós, G. J. Koteles, T. M. Seed

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Abstract

Changes of calcium-content and permeability of tight junction following X- irradiation were investigated in mouse intestinal epithelial cells by electron microscopy. In the control animals the lower parts of tight junctional area as well as the other junctional elements and the intercellular space are labeled by pyroantimonate precipitates, which contain calcium as revealed by electron spectroscopy and electron energy loss spectrometry. X-irradiation, parallel with morphological changes, lead to rapid decrease of pyroantimonate precipitable calcium content and increase of the permeability of light junctions indicated by the penetration of ruthenium red into the intercellular space. These changes were readily reversible following 0,5 Gy doses of irradiation however, they persisted up to 24 hours following 5 Gy irradiation. We conclude that irradiation at the applied doses can transiently destabilize the tight junctions in the epithelial layer of the small intestine, presumably through a calcium dependent mechanism.

Original languageEnglish
Pages (from-to)961-971
Number of pages11
JournalScanning Microscopy
Volume7
Issue number3
Publication statusPublished - Sep 1993

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intestines
Ruthenium
ruthenium
mice
calcium
Calcium
permeability
Irradiation
irradiation
Dosimetry
dosage
Electron spectroscopy
Spectrometry
Electron microscopy
electron spectroscopy
animals
Precipitates
precipitates
Energy dissipation
electron microscopy

Keywords

  • electron energy loss spectrometry
  • electron spectroscopy
  • pyroantimonate precipitable calcium
  • ruthenium red permeability
  • small intestine
  • tight junction
  • X-irradiation

ASJC Scopus subject areas

  • Instrumentation

Cite this

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title = "Morphological and histochemical changes in intercellular junctional complexes in epithelial cells of mouse small intestine upon X-irradiation: Changes of ruthenium red permeability and calcium content",
abstract = "Changes of calcium-content and permeability of tight junction following X- irradiation were investigated in mouse intestinal epithelial cells by electron microscopy. In the control animals the lower parts of tight junctional area as well as the other junctional elements and the intercellular space are labeled by pyroantimonate precipitates, which contain calcium as revealed by electron spectroscopy and electron energy loss spectrometry. X-irradiation, parallel with morphological changes, lead to rapid decrease of pyroantimonate precipitable calcium content and increase of the permeability of light junctions indicated by the penetration of ruthenium red into the intercellular space. These changes were readily reversible following 0,5 Gy doses of irradiation however, they persisted up to 24 hours following 5 Gy irradiation. We conclude that irradiation at the applied doses can transiently destabilize the tight junctions in the epithelial layer of the small intestine, presumably through a calcium dependent mechanism.",
keywords = "electron energy loss spectrometry, electron spectroscopy, pyroantimonate precipitable calcium, ruthenium red permeability, small intestine, tight junction, X-irradiation",
author = "Z. Somosy and J. Kov{\'a}cs and L. Sikl{\'o}s and Koteles, {G. J.} and Seed, {T. M.}",
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TY - JOUR

T1 - Morphological and histochemical changes in intercellular junctional complexes in epithelial cells of mouse small intestine upon X-irradiation

T2 - Changes of ruthenium red permeability and calcium content

AU - Somosy, Z.

AU - Kovács, J.

AU - Siklós, L.

AU - Koteles, G. J.

AU - Seed, T. M.

PY - 1993/9

Y1 - 1993/9

N2 - Changes of calcium-content and permeability of tight junction following X- irradiation were investigated in mouse intestinal epithelial cells by electron microscopy. In the control animals the lower parts of tight junctional area as well as the other junctional elements and the intercellular space are labeled by pyroantimonate precipitates, which contain calcium as revealed by electron spectroscopy and electron energy loss spectrometry. X-irradiation, parallel with morphological changes, lead to rapid decrease of pyroantimonate precipitable calcium content and increase of the permeability of light junctions indicated by the penetration of ruthenium red into the intercellular space. These changes were readily reversible following 0,5 Gy doses of irradiation however, they persisted up to 24 hours following 5 Gy irradiation. We conclude that irradiation at the applied doses can transiently destabilize the tight junctions in the epithelial layer of the small intestine, presumably through a calcium dependent mechanism.

AB - Changes of calcium-content and permeability of tight junction following X- irradiation were investigated in mouse intestinal epithelial cells by electron microscopy. In the control animals the lower parts of tight junctional area as well as the other junctional elements and the intercellular space are labeled by pyroantimonate precipitates, which contain calcium as revealed by electron spectroscopy and electron energy loss spectrometry. X-irradiation, parallel with morphological changes, lead to rapid decrease of pyroantimonate precipitable calcium content and increase of the permeability of light junctions indicated by the penetration of ruthenium red into the intercellular space. These changes were readily reversible following 0,5 Gy doses of irradiation however, they persisted up to 24 hours following 5 Gy irradiation. We conclude that irradiation at the applied doses can transiently destabilize the tight junctions in the epithelial layer of the small intestine, presumably through a calcium dependent mechanism.

KW - electron energy loss spectrometry

KW - electron spectroscopy

KW - pyroantimonate precipitable calcium

KW - ruthenium red permeability

KW - small intestine

KW - tight junction

KW - X-irradiation

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