A kukorica portoktenyészetekben a legnagyobb számú spontán diliaploid növényt eredményezo mikrospóra eredetu struktúra morfológiai és citológiai jellemzése

Translated title of the contribution: Morphological and cytological characterisation of the microspore-derived structure yielding the highest amount of spontaneous doubled haploid regenerants in maize anther cultures

Katalin Jäger, Dávid Koszegi, B. Barnabás

Research output: Contribution to journalArticle

Abstract

The morphological and cytological characteristics of microspore-derived structures produced in anther cultures of maize were studied in order to identify the morphotype resulting in the highest yield of spontaneous doubled haploid regenerants. Parallel to the morphological studies the ploidy level of the microspore-derived structures and regenerants was analysed by flow cytometry. Neither the growth conditions of the anther donor plants nor the media used in the experiment had any effect on the frequency distribution of different morphotypes. The highest number of spontaneous doubled haploid plants was regenerated from white compact structures 2-3 mm in size, derived from the anthers of phytotron-grown donor plants.

Original languageHungarian
Pages (from-to)23-33
Number of pages11
JournalNovenytermeles
Volume54
Issue number1-2
Publication statusPublished - 2005

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anther culture
doubled haploids
microspores
anthers
corn
ploidy
flow cytometry

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

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title = "A kukorica portokteny{\'e}szetekben a legnagyobb sz{\'a}m{\'u} spont{\'a}n diliaploid n{\"o}v{\'e}nyt eredm{\'e}nyezo mikrosp{\'o}ra eredetu strukt{\'u}ra morfol{\'o}giai {\'e}s citol{\'o}giai jellemz{\'e}se",
abstract = "The morphological and cytological characteristics of microspore-derived structures produced in anther cultures of maize were studied in order to identify the morphotype resulting in the highest yield of spontaneous doubled haploid regenerants. Parallel to the morphological studies the ploidy level of the microspore-derived structures and regenerants was analysed by flow cytometry. Neither the growth conditions of the anther donor plants nor the media used in the experiment had any effect on the frequency distribution of different morphotypes. The highest number of spontaneous doubled haploid plants was regenerated from white compact structures 2-3 mm in size, derived from the anthers of phytotron-grown donor plants.",
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author = "Katalin J{\"a}ger and D{\'a}vid Koszegi and B. Barnab{\'a}s",
year = "2005",
language = "Hungarian",
volume = "54",
pages = "23--33",
journal = "Novenytermeles",
issn = "0546-8191",
publisher = "Agroinform",
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TY - JOUR

T1 - A kukorica portoktenyészetekben a legnagyobb számú spontán diliaploid növényt eredményezo mikrospóra eredetu struktúra morfológiai és citológiai jellemzése

AU - Jäger, Katalin

AU - Koszegi, Dávid

AU - Barnabás, B.

PY - 2005

Y1 - 2005

N2 - The morphological and cytological characteristics of microspore-derived structures produced in anther cultures of maize were studied in order to identify the morphotype resulting in the highest yield of spontaneous doubled haploid regenerants. Parallel to the morphological studies the ploidy level of the microspore-derived structures and regenerants was analysed by flow cytometry. Neither the growth conditions of the anther donor plants nor the media used in the experiment had any effect on the frequency distribution of different morphotypes. The highest number of spontaneous doubled haploid plants was regenerated from white compact structures 2-3 mm in size, derived from the anthers of phytotron-grown donor plants.

AB - The morphological and cytological characteristics of microspore-derived structures produced in anther cultures of maize were studied in order to identify the morphotype resulting in the highest yield of spontaneous doubled haploid regenerants. Parallel to the morphological studies the ploidy level of the microspore-derived structures and regenerants was analysed by flow cytometry. Neither the growth conditions of the anther donor plants nor the media used in the experiment had any effect on the frequency distribution of different morphotypes. The highest number of spontaneous doubled haploid plants was regenerated from white compact structures 2-3 mm in size, derived from the anthers of phytotron-grown donor plants.

KW - Maize

KW - Microspore-derived structures

KW - Morphological characteristics

KW - Regeneration efficiency

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JF - Novenytermeles

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