Morphological-anatomical characterization and molecular identification of Tomentella stuposa ectomycorrhizae and related anatomotypes

Erzsébet Jakucs, G. Kovács, R. Agerer, C. Romsics, Z. Eros-Honti

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Species in the genus Tomentella (Thelephoraceae) belong to the most frequent and widespread ectomycorrhizal (EM) fungi found in temperate and boreal forests. Although several unidentified tomentelloid morphotypes have been presented as common members of EM communities in coniferous and broad-leaved forests, few tomentelloid EM have been identified and described in detail. In this study, ten tomentelloid EM isolates collected from Populus alba, Quercus cerris and Picea abies stands in Hungary and Germany are characterized and documented by morphological-anatomical methods using light microscopy. The investigated ectomycorrhizae belong to the same brown-black tomentelloid morphotype but form two different anatomotype groups (At I and At II). Molecular taxonomical identification was accomplished using phylogenetic analysis (neighbor joining method) of 49 Tomentella nrDNA-ITS nucleotide sequences including the 10 new and 39 GenBank sequences. The EM isolates clustered into two adjoining clades identical with the two anatomotypes. At II clustered with Tomentella stuposa while At I could not be identified to species. Based on the morphological similarity and the low genetic difference it must be a closely related taxon. A comparison of the recently known tomentelloid EM to T. stuposa is presented. Ecological questions involving abundance and host relationships are discussed.

Original languageEnglish
Pages (from-to)247-258
Number of pages12
JournalMycorrhiza
Volume15
Issue number4
DOIs
Publication statusPublished - Jun 2005

Fingerprint

Tomentella
Mycorrhizae
ectomycorrhiza
ectomycorrhizae
morphotype
Abies
Picea
Populus
Quercus
broad-leaved forest
Hungary
Nucleic Acid Databases
Thelephoraceae
temperate forest
Quercus cerris
boreal forest
Germany
Populus alba
microscopy
Microscopy

Keywords

  • Ectomycorrhiza
  • ITS sequences
  • Tomentella stuposa

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Plant Science

Cite this

Morphological-anatomical characterization and molecular identification of Tomentella stuposa ectomycorrhizae and related anatomotypes. / Jakucs, Erzsébet; Kovács, G.; Agerer, R.; Romsics, C.; Eros-Honti, Z.

In: Mycorrhiza, Vol. 15, No. 4, 06.2005, p. 247-258.

Research output: Contribution to journalArticle

Jakucs, Erzsébet ; Kovács, G. ; Agerer, R. ; Romsics, C. ; Eros-Honti, Z. / Morphological-anatomical characterization and molecular identification of Tomentella stuposa ectomycorrhizae and related anatomotypes. In: Mycorrhiza. 2005 ; Vol. 15, No. 4. pp. 247-258.
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