Morphologic, host specificity, and molecular characterization of a Hungarian Cryptosporidium meleagridis isolate

T. Sréter, G. Kovács, Alexandre J. Da Silva, Norman J. Pieniazek, Z. Széll, M. Dobos-Kovács, K. Márialigeti, I. Varga

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Abstract

This study was undertaken in order to characterize Cryptosporidium meleagridis isolated from a turkey in Hungary and to compare the morphologies, host specificities, organ locations, and small-subunit RNA (SSU rRNA) gene sequences of this organism and other Cryptosporidium species. The phenotypic differences between C. meleagridis and Cryptosporidium parvum Hungarian calf isolate (zoonotic genotype) oocysts were small, although they were statistically significant. Oocysts of C. meleagridis were successfully passaged in turkeys and were transmitted from turkeys to immunosuppressed mice and from mice to chickens. The location of C. meleagridis was the small intestine, like the location of C. parvum. A comparison of sequence data for the variable region of the SSU rRNA gene of C. meleagridis isolated from turkeys with other Cryptosporidium sequence data in the GenBank database revealed that the Hungarian C. meleagridis sequence is identical to a C. meleagridis sequence recently described for a North Carolina isolate. Thus, C. meleagridis is a distinct species that occurs worldwide and has a broad host range, like the C. parvum zoonotic strain (also called the calf or bovine strain) and Cryptosporidium felis. Because birds are susceptible to C. meleagridis and to some zoonotic strains of C. parvum, these animals may play an active role in contamination of surface waters not only with Cryptosporidium baileyi but also with C. parvum-like parasites.

Original languageEnglish
Pages (from-to)735-738
Number of pages4
JournalApplied and Environmental Microbiology
Volume66
Issue number2
DOIs
Publication statusPublished - Feb 2000

Fingerprint

Cryptosporidium parvum
Cryptosporidium
host specificity
Host Specificity
Zoonoses
gene
host range
Oocysts
parasite
genotype
rRNA Genes
oocysts
bird
surface water
Cryptosporidium baileyi
Felis
ribosomal RNA
animal
calves
Hungary

ASJC Scopus subject areas

  • Environmental Science(all)
  • Biotechnology
  • Microbiology

Cite this

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title = "Morphologic, host specificity, and molecular characterization of a Hungarian Cryptosporidium meleagridis isolate",
abstract = "This study was undertaken in order to characterize Cryptosporidium meleagridis isolated from a turkey in Hungary and to compare the morphologies, host specificities, organ locations, and small-subunit RNA (SSU rRNA) gene sequences of this organism and other Cryptosporidium species. The phenotypic differences between C. meleagridis and Cryptosporidium parvum Hungarian calf isolate (zoonotic genotype) oocysts were small, although they were statistically significant. Oocysts of C. meleagridis were successfully passaged in turkeys and were transmitted from turkeys to immunosuppressed mice and from mice to chickens. The location of C. meleagridis was the small intestine, like the location of C. parvum. A comparison of sequence data for the variable region of the SSU rRNA gene of C. meleagridis isolated from turkeys with other Cryptosporidium sequence data in the GenBank database revealed that the Hungarian C. meleagridis sequence is identical to a C. meleagridis sequence recently described for a North Carolina isolate. Thus, C. meleagridis is a distinct species that occurs worldwide and has a broad host range, like the C. parvum zoonotic strain (also called the calf or bovine strain) and Cryptosporidium felis. Because birds are susceptible to C. meleagridis and to some zoonotic strains of C. parvum, these animals may play an active role in contamination of surface waters not only with Cryptosporidium baileyi but also with C. parvum-like parasites.",
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AU - Kovács, G.

AU - Da Silva, Alexandre J.

AU - Pieniazek, Norman J.

AU - Széll, Z.

AU - Dobos-Kovács, M.

AU - Márialigeti, K.

AU - Varga, I.

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