Monoclonal antibody-recognizing cone visual pigment

A. Szél, L. Takács, E. Monostori, T. Diamantstein, I. Vigh-Teichmann, P. Röhlich

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

Monoclonal antibodies were raised to a crude photoreceptor-membrane suspension from chicken retinas. Clones producing antibodies against cone outer segments were selected by screening with immunocytochemistry on semithin sections of the retina. One monoclonal antibody, called COS-1, specifically labelled outer segments of double cones and one type of single cones; outer segments of rods and several single cones were not stained. On immunoblots of retinal photoreceptor membranes, this antibody recognized a protein with an apparent molecular mass of 33000. The visual pigment character of the 33000 protein was indirectly established by another monoclonal antibody, OS-2, which labelled all outer segments on semithin sections and four bands (33000-, 36000-, 38000-40000- and a composite band between 66000-72000 MW) on immunoblots. Of these, the 36000- and the 72000 MW protein bands were identified with an anti-rhodopsin polyclonal antibody as rhodopsin monomer and dimer. Monoclonal antibody OS-2 is assumed therefore to represent an antibody against a common epitope of all visual pigments of the chicken. The monoclonal antibody COS-1 was found to bind to certain cone outer segments of many other vertebrate species as well.

Original languageEnglish
Pages (from-to)871-883
Number of pages13
JournalExperimental Eye Research
Volume43
Issue number6
DOIs
Publication statusPublished - 1986

Fingerprint

Retinal Pigments
Monoclonal Antibodies
Rhodopsin
Antibodies
Retina
Chickens
Rod Cell Outer Segment
Vertebrate Photoreceptor Cells
Proteins
Membranes
Vertebrates
Epitopes
Suspensions
Clone Cells
Immunohistochemistry

Keywords

  • chicken retina
  • cone
  • monoclonal antibody
  • visual pigment

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems

Cite this

Monoclonal antibody-recognizing cone visual pigment. / Szél, A.; Takács, L.; Monostori, E.; Diamantstein, T.; Vigh-Teichmann, I.; Röhlich, P.

In: Experimental Eye Research, Vol. 43, No. 6, 1986, p. 871-883.

Research output: Contribution to journalArticle

Szél, A. ; Takács, L. ; Monostori, E. ; Diamantstein, T. ; Vigh-Teichmann, I. ; Röhlich, P. / Monoclonal antibody-recognizing cone visual pigment. In: Experimental Eye Research. 1986 ; Vol. 43, No. 6. pp. 871-883.
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