Monoclonal antibody based electrochemical immunosensor for the determination of ochratoxin A in wheat

Sergio Hugo Alarcón, Giuseppe Palleschi, Dario Compagnone, Michelangelo Pascale, Angelo Visconti, Ildikó Barna-Vetró

Research output: Contribution to journalArticle

94 Citations (Scopus)

Abstract

Competitive electrochemical enzyme-linked immunosorbent assays based on disposable screen-printed electrodes have been developed for quantitative determination of ochratoxin A (OTA). The assays were carried out using monoclonal antibodies in the direct and indirect format. OTA working range, I50 and detection limits were 0.05-2.5 and 0.1-7.5 μg L-1, 0.35 (±0.04) μg L-1 and 0.9 (±0.1) μg L-1, 60 and 100 μg L-1 in the direct and indirect assay format, respectively. The immunosensor in the direct format was selected for the determination of OTA in wheat. Samples were extracted with aqueous acetonitrile and the extract analyzed directly by the assay without clean-up. The I50 in real samples was 0.2 μg L-1 corresponding to 1.6 μg/kg in the wheat sample with a detection limit of 0.4 μg/kg (calculated as blank signal -3σ). Within- and between-assay variability were less than 5 and 10%, respectively. A good correlation (r = 0.9992) was found by comparative analysis of naturally contaminated wheat samples using this assay and an HPLC/immunoaffinity clean-up method based on the AOAC Official Method 2000.03 for the determination of OTA in barley.

Original languageEnglish
Pages (from-to)1031-1037
Number of pages7
JournalTalanta
Volume69
Issue number4
DOIs
Publication statusPublished - Jun 15 2006

Keywords

  • ELISA
  • Immunosensors
  • Ochratoxin A
  • Screen-printed electrodes
  • Wheat

ASJC Scopus subject areas

  • Analytical Chemistry

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