Monoclonal antibodies identify four distinct annelid leukocyte markers

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

This paper describes for the first time the production and characterization of a library of monoclonal antibodies (anti-EFCC clones) raised against coelomocyte (leukocyte) markers of Eisenia fetida earthworm. Leukocyte subgroups are components of earthworm innate immunity that require a more precise characterization using immunological markers. Flow cytometry, immunocytochemistry, and immunoprecipitation analyzed and confirmed the specificity of anti-EFCC clones. Anti-EFCC mAbs revealed different leukocyte subpopulations and various staining patterns on tissues. Two functional assays (e.g. phagocytosis and encapsulation) further characterized EFCC clusters revealing a common coelomocyte marker and three subpopulation-specific markers. No crossreactivity was found on human, mouse, rat or cells from Drosophila melanogaster but immunoreactivity was detected on snail (Planorbarius corneus) tissues. Immunohistochemical results suggest mesodermal origin of all coelomocyte subgroups that agree with classical morphological analyses.

Original languageEnglish
Pages (from-to)599-614
Number of pages16
JournalDevelopmental and Comparative Immunology
Volume29
Issue number7
DOIs
Publication statusPublished - 2005

Fingerprint

Oligochaeta
Leukocytes
Monoclonal Antibodies
Clone Cells
Snails
Drosophila melanogaster
Phagocytosis
Immunoprecipitation
Innate Immunity
Flow Cytometry
Immunohistochemistry
Staining and Labeling

Keywords

  • Coelomocytes
  • Encapsulation
  • Flow cytometry
  • Immunocytochemistry
  • Innate immunity
  • Monoclonal antibodies
  • Phagocytosis
  • Western-blot

ASJC Scopus subject areas

  • Developmental Biology
  • Immunology

Cite this

Monoclonal antibodies identify four distinct annelid leukocyte markers. / Engelmann, Péter; Pálinkás, László; Cooper, Edwin L.; Németh, Péter.

In: Developmental and Comparative Immunology, Vol. 29, No. 7, 2005, p. 599-614.

Research output: Contribution to journalArticle

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