Monoclonal antibodies detecting components of the bovine immune system in formaldehyde-fixed paraffin-embedded tissue specimens

G. Keresztes, L. Takács, P. Vilmos, E. Kurucz, I. Andó

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Although the use of monoclonal antibodies in the diagnosis of fixed human material is a daily routine, the lack of suitable reagents recognizing epitopes resistant to formaldehyde fixation is an obvious limit to extending this approach to veterinary research and practice. To find reagents that retain their binding capacity to the recognized epitopes in formaldehyde-fixed samples, bovine lymphoid tissue sections were immunostained using various antigen retrieval procedures with monoclonal antibodies raised against ruminant leukocyte cell surface molecules. As a result, a set of antibodies could be established that allowed the identification of different immune cell types including all or a distinct subpopulation of B and T lymphocytes, monocytes, macrophages, granulcytes, red blood cells, and vascular endothelial cells.

Original languageEnglish
Pages (from-to)383-392
Number of pages10
JournalVeterinary Immunology and Immunopathology
Volume52
Issue number4
DOIs
Publication statusPublished - Aug 1996

Fingerprint

formaldehyde
Paraffin
alkanes
Formaldehyde
epitopes
immune system
Epitopes
Immune System
monoclonal antibodies
Monoclonal Antibodies
cattle
Ruminants
binding capacity
Lymphoid Tissue
blood vessels
monocytes
endothelial cells
B-lymphocytes
Monocytes
ruminants

Keywords

  • Cattle
  • Formaldehyde fixation
  • Immune system
  • Immunohistochemistry

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Immunology
  • veterinary(all)

Cite this

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AU - Takács, L.

AU - Vilmos, P.

AU - Kurucz, E.

AU - Andó, I.

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AB - Although the use of monoclonal antibodies in the diagnosis of fixed human material is a daily routine, the lack of suitable reagents recognizing epitopes resistant to formaldehyde fixation is an obvious limit to extending this approach to veterinary research and practice. To find reagents that retain their binding capacity to the recognized epitopes in formaldehyde-fixed samples, bovine lymphoid tissue sections were immunostained using various antigen retrieval procedures with monoclonal antibodies raised against ruminant leukocyte cell surface molecules. As a result, a set of antibodies could be established that allowed the identification of different immune cell types including all or a distinct subpopulation of B and T lymphocytes, monocytes, macrophages, granulcytes, red blood cells, and vascular endothelial cells.

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KW - Immunohistochemistry

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