Monoamine precursor uptake in ganglia of a mollusc Anodonta cygnea L. (Bivalvia) - An autoradiographic and ultrastructural study

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Abstract

In vitro-uptake and localization of 3H-5-hydroxytryptophan and 3H-dihydroxyphenylalanine have been investigated by means of light microscopic autoradiography in the central nervous system of the freshwater mussel, Anodonta cygnea L. Accumulation of the labelled monoamine precursors could be observed both over neuronal perikarya and neuropil in the ganglia. 3H-5-hydroxytryptophan and 3H-dihydroxyphenylalanine were taken up by neurons characterized by a small size (10-20 μm), a polygonal form and an intense staining with toluidine blue. Labelled axon branches situated either in the neuropil or between nerve cells in the cortex exhibited only a moderate activity, frequently of a diffuse character. After pretreatment for 24 h with colchicine, silver grains were also seen over the neuronal perikarya and some axon profiles. This shows that local uptake of the monoamine precursors and, presumably, the synthesis of the monoamines takes place both at perikaryal and axonal/terminal level. The nerve cells accumulating the monoamine precursors contain a nucleus with rich chromatin content and a deeply invaginated nuclear envelope. Further ultrastructural characteristics of these neurons are their well-developed rough endoplasmic reticulum system, a great number of mitochondria and glycogen granules, and the presence of dense-cored vesicles of different types. All these features are responsible for the electron-dense appearance of the cytoplasm.

Original languageEnglish
Pages (from-to)403-415
Number of pages13
JournalCell and Tissue Research
Volume219
Issue number2
DOIs
Publication statusPublished - Aug 1981

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Anodonta
Molluscs
Mollusca
Bivalvia
Ganglia
Neurons
5-Hydroxytryptophan
Dihydroxyphenylalanine
Neuropil
Axons
Tolonium Chloride
Mitochondria
Rough Endoplasmic Reticulum
Nuclear Envelope
Colchicine
Neurology
Fresh Water
Autoradiography
Glycogen
Silver

Keywords

  • Autoradiography
  • Ganglia
  • Molluscs
  • Monoamine precursors

ASJC Scopus subject areas

  • Anatomy
  • Clinical Biochemistry
  • Cell Biology

Cite this

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abstract = "In vitro-uptake and localization of 3H-5-hydroxytryptophan and 3H-dihydroxyphenylalanine have been investigated by means of light microscopic autoradiography in the central nervous system of the freshwater mussel, Anodonta cygnea L. Accumulation of the labelled monoamine precursors could be observed both over neuronal perikarya and neuropil in the ganglia. 3H-5-hydroxytryptophan and 3H-dihydroxyphenylalanine were taken up by neurons characterized by a small size (10-20 μm), a polygonal form and an intense staining with toluidine blue. Labelled axon branches situated either in the neuropil or between nerve cells in the cortex exhibited only a moderate activity, frequently of a diffuse character. After pretreatment for 24 h with colchicine, silver grains were also seen over the neuronal perikarya and some axon profiles. This shows that local uptake of the monoamine precursors and, presumably, the synthesis of the monoamines takes place both at perikaryal and axonal/terminal level. The nerve cells accumulating the monoamine precursors contain a nucleus with rich chromatin content and a deeply invaginated nuclear envelope. Further ultrastructural characteristics of these neurons are their well-developed rough endoplasmic reticulum system, a great number of mitochondria and glycogen granules, and the presence of dense-cored vesicles of different types. All these features are responsible for the electron-dense appearance of the cytoplasm.",
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T1 - Monoamine precursor uptake in ganglia of a mollusc Anodonta cygnea L. (Bivalvia) - An autoradiographic and ultrastructural study

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N2 - In vitro-uptake and localization of 3H-5-hydroxytryptophan and 3H-dihydroxyphenylalanine have been investigated by means of light microscopic autoradiography in the central nervous system of the freshwater mussel, Anodonta cygnea L. Accumulation of the labelled monoamine precursors could be observed both over neuronal perikarya and neuropil in the ganglia. 3H-5-hydroxytryptophan and 3H-dihydroxyphenylalanine were taken up by neurons characterized by a small size (10-20 μm), a polygonal form and an intense staining with toluidine blue. Labelled axon branches situated either in the neuropil or between nerve cells in the cortex exhibited only a moderate activity, frequently of a diffuse character. After pretreatment for 24 h with colchicine, silver grains were also seen over the neuronal perikarya and some axon profiles. This shows that local uptake of the monoamine precursors and, presumably, the synthesis of the monoamines takes place both at perikaryal and axonal/terminal level. The nerve cells accumulating the monoamine precursors contain a nucleus with rich chromatin content and a deeply invaginated nuclear envelope. Further ultrastructural characteristics of these neurons are their well-developed rough endoplasmic reticulum system, a great number of mitochondria and glycogen granules, and the presence of dense-cored vesicles of different types. All these features are responsible for the electron-dense appearance of the cytoplasm.

AB - In vitro-uptake and localization of 3H-5-hydroxytryptophan and 3H-dihydroxyphenylalanine have been investigated by means of light microscopic autoradiography in the central nervous system of the freshwater mussel, Anodonta cygnea L. Accumulation of the labelled monoamine precursors could be observed both over neuronal perikarya and neuropil in the ganglia. 3H-5-hydroxytryptophan and 3H-dihydroxyphenylalanine were taken up by neurons characterized by a small size (10-20 μm), a polygonal form and an intense staining with toluidine blue. Labelled axon branches situated either in the neuropil or between nerve cells in the cortex exhibited only a moderate activity, frequently of a diffuse character. After pretreatment for 24 h with colchicine, silver grains were also seen over the neuronal perikarya and some axon profiles. This shows that local uptake of the monoamine precursors and, presumably, the synthesis of the monoamines takes place both at perikaryal and axonal/terminal level. The nerve cells accumulating the monoamine precursors contain a nucleus with rich chromatin content and a deeply invaginated nuclear envelope. Further ultrastructural characteristics of these neurons are their well-developed rough endoplasmic reticulum system, a great number of mitochondria and glycogen granules, and the presence of dense-cored vesicles of different types. All these features are responsible for the electron-dense appearance of the cytoplasm.

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