Monoamine oxidases are novel sources of cardiovascular oxidative stress in experimental diabetes

Adrian Sturza, Oana M. Duicu, Adrian Vaduva, Maria D. Dănilă, Lavinia Noveanu, A. Varró, Danina M. Muntean

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Diabetes mellitus (DM) is widely recognized as the most severe metabolic disease associated with increased cardiovascular morbidity and mortality. The generation of reactive oxygen species (ROS) is a major event causally linked to the development of cardiovascular complications throughout the evolution of DM. Recently, monoamine oxidases (MAOs) at the outer mitochondrial membrane, with 2 isoforms, MAO-A and MAO-B, have emerged as novel sources of constant hydrogen peroxide (H2O2) production in the cardiovascular system via the oxidative deamination of biogenic amines and neurotransmitters. Whether MAOs are mediators of endothelial dysfunction in DM is unknown, and so we studied this in a streptozotocininduced rat model of diabetes. MAO expression (mRNA and protein) was increased in both arterial samples and hearts isolated from the diabetic animals. Also, H2O2 production (ferrous oxidation – xylenol orange assay) in aortic samples was significantly increased, together with an impairment of endothelium-dependent relaxation (organ-bath studies). MAO inhibitors (clorgyline and selegiline) attenuated ROS production by 50% and partially normalized the endothelium-dependent relaxation in diseased vessels. In conclusion, MAOs, in particular the MAO-B isoform, are induced in aortas and hearts in the streptozotocin-induced diabetic rat model and contribute, via the generation of H2O2, to the endothelial dysfunction associated with experimental diabetes.

Original languageEnglish
Pages (from-to)555-561
Number of pages7
JournalCanadian Journal of Physiology and Pharmacology
Volume93
Issue number7
DOIs
Publication statusPublished - Mar 11 2015

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Monoamine Oxidase
Oxidative Stress
Diabetes Mellitus
Endothelium
Reactive Oxygen Species
Protein Isoforms
Clorgyline
Selegiline
Deamination
Biogenic Amines
Monoamine Oxidase Inhibitors
Metabolic Diseases
Mitochondrial Membranes
Streptozocin
Cardiovascular System
Baths
Hydrogen Peroxide
Neurotransmitter Agents
Aorta
Morbidity

Keywords

  • Clorgyline
  • Endothelial dysfunction
  • Experimental diabetes mellitus
  • Monoamine oxidases
  • Oxidative stress
  • Selegiline

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)
  • Pharmacology

Cite this

Monoamine oxidases are novel sources of cardiovascular oxidative stress in experimental diabetes. / Sturza, Adrian; Duicu, Oana M.; Vaduva, Adrian; Dănilă, Maria D.; Noveanu, Lavinia; Varró, A.; Muntean, Danina M.

In: Canadian Journal of Physiology and Pharmacology, Vol. 93, No. 7, 11.03.2015, p. 555-561.

Research output: Contribution to journalArticle

Sturza, Adrian ; Duicu, Oana M. ; Vaduva, Adrian ; Dănilă, Maria D. ; Noveanu, Lavinia ; Varró, A. ; Muntean, Danina M. / Monoamine oxidases are novel sources of cardiovascular oxidative stress in experimental diabetes. In: Canadian Journal of Physiology and Pharmacology. 2015 ; Vol. 93, No. 7. pp. 555-561.
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