Monoamine innervation of bed nucleus of stria terminalis

An electron microscopic investigation

Clyde F. Phelix, Z. Liposits, Willis K. Paull

Research output: Contribution to journalArticle

104 Citations (Scopus)

Abstract

Immunocytochemical studies showed distinctive monoamine input to the bed nucleus of the stria terminalis (BST). A comparison of axons immunoreactive (IR) for a catecholamine synthetic enzyme [tyrosine hydroxylase (TH) or dopamine β-hydroxylase (DBH) or phenylethanolamine-N-methyl transferase (PNMT)] or serotonin (5-HT) was performed. TH-IR axons had a greater density in the lateral BST, but DBH-IR and 5-HT-IR axons had a greater density in the medial BST. PNMT-IR axons were dense in the intermediate BST. TH-IR axons had a greater density than DBH- and PNMT-IR axons in the dorsolateral BST, but DBH-IR axons had the greatest density in the ventrolateral BST. Ultrastructural studies revealed that TH-IR terminals formed synapses with soma, dendrites, spines, and axons in the dorsolateral BST. DBH-IR terminals formed synapses with dendritic shafts and spines, and 5-HT-IR terminals formed synapses with dendrites in the ventrolateral BST. Only some 5-HT-IR axons were myelinated. The medial vs. lateral organization of the noradrenergic and dopaminergic afferents in the BST of the rat brain is now evident and is similar to the human brain. The medial-lateral functional subdivision of the BST is supported by the pattern of dopaminergic, noradrenergic, and serotonergic afferents. This demonstration of epinephrine-producing afferents in the BST is the first detailed description of adrenergic input to the BST and aided the determination that catecholaminergic innervation of the ventrolateral BST is predominantly noradrenergic as has been proposed for many years. However, the additional demonstration of rich dopaminergic innervation of the dorsolateral subnucleus suggests further division of the BST into dorsal and ventral functional subgroups.

Original languageEnglish
Pages (from-to)949-965
Number of pages17
JournalBrain Research Bulletin
Volume28
Issue number6
DOIs
Publication statusPublished - 1992

Fingerprint

Septal Nuclei
Electrons
Axons
Tyrosine 3-Monooxygenase
Serotonin
Transferases
Synapses
Dendrites
Dendritic Spines
Brain
Carisoprodol
Mixed Function Oxygenases
Adrenergic Agents
Epinephrine
Catecholamines

Keywords

  • Bed nucleus of stria terminalis Catecholamines Serotonin Tyrosine hydroxylase Dopamine β-hydroxylase Phenylethanolamine N-methyl transferase Immunocytochemistry Electron microscopy Ultrastructure Quantitative morphometry

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Monoamine innervation of bed nucleus of stria terminalis : An electron microscopic investigation. / Phelix, Clyde F.; Liposits, Z.; Paull, Willis K.

In: Brain Research Bulletin, Vol. 28, No. 6, 1992, p. 949-965.

Research output: Contribution to journalArticle

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