Monitoring of transcriptional responses in roots of six wheat cultivars during mild drought stress

M. Szécsényi, M. Cserháti, A. Zvara, D. Dudits, J. Györgyey

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

In order to study drought stress responses in roots during moderate water shortage, our group has designed an oligonucleotide microarray containing probes for genes annotated as either stress-related or general ones to examine six wheat cultivars. These include two drought-tolerant and four drought-sensitive genotypes, having different root morphology either during optimal or water-deficit stress conditions. After a two-week-long water withdrawal, no significant changes were detected in root weights but various trends were observed in the cultivars. These tendencies showed relevant correlations with expression kinetics of genes encoding proteins related to root growth and development. On the other hand, probes indicating variation in distinct genotypes mainly belonged to the group of stress-related genes which encode proteins that contribute to carbohydrate remodeling, osmoprotectant accumulation, amino acid metabolism, defense against oxidative stress, and regulation of transcription. Hence, these data allow us to focus on major metabolic processes involved in drought adaptation as well as provide a wide selection of candidate genes for further detailed studies.

Original languageEnglish
Pages (from-to)527-538
Number of pages12
JournalCereal Research Communications
Volume41
Issue number4
DOIs
Publication statusPublished - Dec 1 2013

Fingerprint

Droughts
Triticum
water stress
wheat
monitoring
drought
cultivars
genes
Genotype
Genes
osmotolerance
Water
water shortages
genotype
amino acid metabolism
oligonucleotides
Oligonucleotide Array Sequence Analysis
Growth and Development
Dehydration
stress response

Keywords

  • drought
  • oligonucleotide-microarray
  • root
  • transcriptome changes
  • wheat

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Physiology

Cite this

Monitoring of transcriptional responses in roots of six wheat cultivars during mild drought stress. / Szécsényi, M.; Cserháti, M.; Zvara, A.; Dudits, D.; Györgyey, J.

In: Cereal Research Communications, Vol. 41, No. 4, 01.12.2013, p. 527-538.

Research output: Contribution to journalArticle

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