Monitoring of the conjugation reaction between human serum transferrin and fluorescein isothiocyanate by capillary electrophoresis

Tünde Konecsni, Ferenc Kilár

Research output: Contribution to journalConference article

11 Citations (Scopus)

Abstract

Labeling of iron-free human serum transferrin by an amine-reactive probe, fluorescein isothiocyanate (FITC) was monitored with different dye to protein ratios. The degree of labeling was followed and determined by capillary electrophoresis. Depending on the number of the bound FITC, a shift in the electropherogram was observed, but the conjugated-transferrin forms were resolved from the unbound FITC in zone electrophoresis. The lowest protein concentration that resulted in detectable transferrin conjugate was 0.13 μM, and the limit of detection of the conjugated protein was 0.13 nM (10 ng/ml) with a signal to noise ratio of one to three.

Original languageEnglish
Pages (from-to)135-139
Number of pages5
JournalJournal of Chromatography A
Volume1051
Issue number1-2
DOIs
Publication statusPublished - Oct 8 2004
Event17th International Symposium on Microscale Separations - Salzburg, Austria
Duration: Feb 8 2004Feb 12 2004

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Keywords

  • Derivatization, electrophoresis
  • Fluorescein isothiocyanate
  • Glycoproteins
  • Proteins
  • Transferrin

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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