Monitoring of selected metabolites and biotransformation products from fermentation broths by high-performance liquid chromatography

György Morovján, György Szakács, Jenö Fekete

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Reversed-phase high-performance liquid chromatographic methods with UV-photodiode array or multiwavelength detection were developed for the analysis of secondary fungal metabolites mevinolin (also known as monacolin K, lovastatin or Mevacor) and fumagillin in fermentation broths. Samples obtained from screening experiments of Aspergillus fumigatus and Aspergillus terreus strains were analyzed for fumagillin and mevinolin, respectively. Isocratic and gradient elution was used with acetonitrile-aqueous phosphoric acid or acetonitrile-phosphate buffer eluents. Sample preparation was accomplished by extraction of the whole fermentation broth with an acid-organic solvent mixture. Similarly, bacterial degradation products of 2,4,6-trichlorophenol obtained from microbial remediation processes were analyzed by reversed-phase HPLC with UV-photodiode array detection. Extraction of the analyte from solid samples was done by solvent extraction and solid-phase extraction clean-up using octadecyl-modified silica sample preparation cartridges.

Original languageEnglish
Pages (from-to)165-172
Number of pages8
JournalJournal of Chromatography A
Volume763
Issue number1-2
DOIs
Publication statusPublished - Feb 28 1997

    Fingerprint

Keywords

  • Aspergillus spp
  • Fermentation broth
  • Fumagillin
  • Mevinolin
  • Trichlorophenol

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

Cite this