Monitoring of haploid maize cell suspension culture conditions in bioreactors

Géza Kevács, Miklós László, György Rajkai, B. Barnabás

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Maize (Zea mays L.) haploid cells were cultivated in a 1500 ml aerated and stirred batch bioreactor using modified BM medium. Cell growth was highly affected by pH and dissolved oxygen, and we observed two fairly distinct growth phases. During the first two days after inoculation at pH 5.8, oxygen consumption was high and the cells lowered the pH to a value around 4.3. After this period the pH stabilized at 4.5 and the dissolved oxygen reached a steady level. Decreasing dissolved oxygen concentration leads to lower growth rate and to higher pH. Both events mean stress conditions for the cell culture and probably result in increased genetic variability, and the loss of regeneration capacity. The stress condition during the adaptation phase can be eliminated by decreasing the pH of the medium to 4.7 before inoculation and by keeping dissolved oxygen above 40%. These conditions provide prolonged exponential growth dynamics and the cell suspensions could be the basis of large scale cultures also.

Original languageEnglish
Pages (from-to)123-126
Number of pages4
JournalPlant Cell, Tissue and Organ Culture
Volume43
Issue number2
DOIs
Publication statusPublished - Nov 1995

Fingerprint

Haploidy
Bioreactors
bioreactors
haploidy
cell suspension culture
Zea mays
Suspensions
Cell Culture Techniques
dissolved oxygen
corn
monitoring
Oxygen
Growth
Oxygen Consumption
oxygen consumption
Regeneration
cell growth
cell culture
cells
genetic variation

Keywords

  • bioreactor
  • dissolved oxygen
  • haploid cell suspension
  • pH
  • Zea mays

ASJC Scopus subject areas

  • Biotechnology
  • Plant Science

Cite this

Monitoring of haploid maize cell suspension culture conditions in bioreactors. / Kevács, Géza; László, Miklós; Rajkai, György; Barnabás, B.

In: Plant Cell, Tissue and Organ Culture, Vol. 43, No. 2, 11.1995, p. 123-126.

Research output: Contribution to journalArticle

Kevács, Géza ; László, Miklós ; Rajkai, György ; Barnabás, B. / Monitoring of haploid maize cell suspension culture conditions in bioreactors. In: Plant Cell, Tissue and Organ Culture. 1995 ; Vol. 43, No. 2. pp. 123-126.
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