Monitoring by HPLC of chamomile flavonoids exposed to rat liver microsomal metabolism

Georg Petroianu, E. Szőke, H. Kalász, Péter Szegi, Rudolf Laufer, Bernadett Benkco, Ferenc Darvas, K. Tekes

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Three major flavonoid chamomile components (quercetin, apigenin-7-O- glucoside and rutin) were subjected to oxidative metabolism by cytochrome P-450 of rat liver microsomal preparations. Changes over time in their respective concentrations were followed using reversed-phase HPLC with UV detection. No clean-up had to be applied as only the specific flavonoid had to be separated from the background components originating from the rat liver microsome. Neither the concentration of apigenin-7-O-glucoside nor that of the diglycoside rutin decreased during one hour of exposure to rat microsomal treatment. In contrast, the concentration of quercetin, a lipophilic aglycon, decreased. Our analytical HPLC results complement the in silico calculated lipophilicity (logP) of these compounds; the relatively high lipophilicity of quercetin appears to predispose it to oxidative metabolism in order to decrease its fat solubility. In contrast the much less lipophilic compounds apigenin-7-O-glucoside and rutin were resistant in vitro to microsomal treatment.

Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalOpen Medicinal Chemistry Journal
Volume3
DOIs
Publication statusPublished - 2009

Fingerprint

Chamomile
Rutin
Flavonoids
Quercetin
High Pressure Liquid Chromatography
Liver
Liver Microsomes
Computer Simulation
Solubility
Cytochrome P-450 Enzyme System
Fats
apigetrin

Keywords

  • Apigenin-7-O-glucoside
  • Cytochrome P-450 dependent oxidation
  • HPLC
  • Microsomal treatment
  • Quercetin
  • Rutin

ASJC Scopus subject areas

  • Pharmacology
  • Drug Discovery
  • Molecular Medicine
  • Pharmaceutical Science

Cite this

Monitoring by HPLC of chamomile flavonoids exposed to rat liver microsomal metabolism. / Petroianu, Georg; Szőke, E.; Kalász, H.; Szegi, Péter; Laufer, Rudolf; Benkco, Bernadett; Darvas, Ferenc; Tekes, K.

In: Open Medicinal Chemistry Journal, Vol. 3, 2009, p. 1-7.

Research output: Contribution to journalArticle

Petroianu, Georg ; Szőke, E. ; Kalász, H. ; Szegi, Péter ; Laufer, Rudolf ; Benkco, Bernadett ; Darvas, Ferenc ; Tekes, K. / Monitoring by HPLC of chamomile flavonoids exposed to rat liver microsomal metabolism. In: Open Medicinal Chemistry Journal. 2009 ; Vol. 3. pp. 1-7.
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