Molecular typing of human herpesvirus 8 isolates from patients with Kaposi's sarcoma in Hungary

Rita Ötvös, A. Juhász, Erika Szalai, Dorina Ujvari, Katalin Ötvös, Katalin Szabo, E. Remenyik, Laszlo Szekely, L. Gergely, Jozsef Konya

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: Kaposi's sarcoma (KS) shows a distinct geographical and ethnic distribution. Genes at both ends of the human herpesvirus 8 (HHV-8) genome have been shown to vary considerably. Seven major molecular subtypes of HHV-8 were defined based on the amino acid sequence of the open reading frame K1 (orf-K1). The aim of the present study was to characterize HHV8 isolates from hospitalized patients in Hungary. Materials and Methods: A total of 36 archival paraffin-embedded Kaposi's sarcoma tissue samples were collected. Polymerase chain reaction (PCR) was carried out on the extracted DNA, using specific primers for HHV-8. After identifying the presence of HHV-8 by amplification of its orf26 region, the orf-K1 region was amplified, sequenced and used for phylogenetic analysis. Results: From the 36 orf26-positive cases, orf-K1 was amplified and was analyzed successfully in 12 cases. Phylogenetic studies, based on the complete K1 gene/protein sequences, indicate that all strains belong to the A subtype. Specifically, six of them were related to the A1 subgroup, six to the A2 subgroup and three previously reported to the A3 subgroup. Nucleotide sequence data are reported and are available in the Genbank database under accession numbers KF829938-KF829947.

Original languageEnglish
Pages (from-to)893-898
Number of pages6
JournalAnticancer Research
Volume34
Issue number2
Publication statusPublished - Feb 1 2014

Fingerprint

Molecular Typing
Human Herpesvirus 8
Hungary
Kaposi's Sarcoma
Open Reading Frames
Nucleic Acid Databases
varespladib methyl
Paraffin
Amino Acid Sequence
Genome
Databases
Polymerase Chain Reaction
DNA
Genes
Proteins

Keywords

  • HHV-8 genotypes
  • K1
  • Kaposi's sarcoma
  • PCR
  • Subtype A

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Ötvös, R., Juhász, A., Szalai, E., Ujvari, D., Ötvös, K., Szabo, K., ... Konya, J. (2014). Molecular typing of human herpesvirus 8 isolates from patients with Kaposi's sarcoma in Hungary. Anticancer Research, 34(2), 893-898.

Molecular typing of human herpesvirus 8 isolates from patients with Kaposi's sarcoma in Hungary. / Ötvös, Rita; Juhász, A.; Szalai, Erika; Ujvari, Dorina; Ötvös, Katalin; Szabo, Katalin; Remenyik, E.; Szekely, Laszlo; Gergely, L.; Konya, Jozsef.

In: Anticancer Research, Vol. 34, No. 2, 01.02.2014, p. 893-898.

Research output: Contribution to journalArticle

Ötvös, R, Juhász, A, Szalai, E, Ujvari, D, Ötvös, K, Szabo, K, Remenyik, E, Szekely, L, Gergely, L & Konya, J 2014, 'Molecular typing of human herpesvirus 8 isolates from patients with Kaposi's sarcoma in Hungary', Anticancer Research, vol. 34, no. 2, pp. 893-898.
Ötvös R, Juhász A, Szalai E, Ujvari D, Ötvös K, Szabo K et al. Molecular typing of human herpesvirus 8 isolates from patients with Kaposi's sarcoma in Hungary. Anticancer Research. 2014 Feb 1;34(2):893-898.
Ötvös, Rita ; Juhász, A. ; Szalai, Erika ; Ujvari, Dorina ; Ötvös, Katalin ; Szabo, Katalin ; Remenyik, E. ; Szekely, Laszlo ; Gergely, L. ; Konya, Jozsef. / Molecular typing of human herpesvirus 8 isolates from patients with Kaposi's sarcoma in Hungary. In: Anticancer Research. 2014 ; Vol. 34, No. 2. pp. 893-898.
@article{7be6921988f543aa8b263d422ae0d701,
title = "Molecular typing of human herpesvirus 8 isolates from patients with Kaposi's sarcoma in Hungary",
abstract = "Background: Kaposi's sarcoma (KS) shows a distinct geographical and ethnic distribution. Genes at both ends of the human herpesvirus 8 (HHV-8) genome have been shown to vary considerably. Seven major molecular subtypes of HHV-8 were defined based on the amino acid sequence of the open reading frame K1 (orf-K1). The aim of the present study was to characterize HHV8 isolates from hospitalized patients in Hungary. Materials and Methods: A total of 36 archival paraffin-embedded Kaposi's sarcoma tissue samples were collected. Polymerase chain reaction (PCR) was carried out on the extracted DNA, using specific primers for HHV-8. After identifying the presence of HHV-8 by amplification of its orf26 region, the orf-K1 region was amplified, sequenced and used for phylogenetic analysis. Results: From the 36 orf26-positive cases, orf-K1 was amplified and was analyzed successfully in 12 cases. Phylogenetic studies, based on the complete K1 gene/protein sequences, indicate that all strains belong to the A subtype. Specifically, six of them were related to the A1 subgroup, six to the A2 subgroup and three previously reported to the A3 subgroup. Nucleotide sequence data are reported and are available in the Genbank database under accession numbers KF829938-KF829947.",
keywords = "HHV-8 genotypes, K1, Kaposi's sarcoma, PCR, Subtype A",
author = "Rita {\"O}tv{\"o}s and A. Juh{\'a}sz and Erika Szalai and Dorina Ujvari and Katalin {\"O}tv{\"o}s and Katalin Szabo and E. Remenyik and Laszlo Szekely and L. Gergely and Jozsef Konya",
year = "2014",
month = "2",
day = "1",
language = "English",
volume = "34",
pages = "893--898",
journal = "Anticancer Research",
issn = "0250-7005",
publisher = "International Institute of Anticancer Research",
number = "2",

}

TY - JOUR

T1 - Molecular typing of human herpesvirus 8 isolates from patients with Kaposi's sarcoma in Hungary

AU - Ötvös, Rita

AU - Juhász, A.

AU - Szalai, Erika

AU - Ujvari, Dorina

AU - Ötvös, Katalin

AU - Szabo, Katalin

AU - Remenyik, E.

AU - Szekely, Laszlo

AU - Gergely, L.

AU - Konya, Jozsef

PY - 2014/2/1

Y1 - 2014/2/1

N2 - Background: Kaposi's sarcoma (KS) shows a distinct geographical and ethnic distribution. Genes at both ends of the human herpesvirus 8 (HHV-8) genome have been shown to vary considerably. Seven major molecular subtypes of HHV-8 were defined based on the amino acid sequence of the open reading frame K1 (orf-K1). The aim of the present study was to characterize HHV8 isolates from hospitalized patients in Hungary. Materials and Methods: A total of 36 archival paraffin-embedded Kaposi's sarcoma tissue samples were collected. Polymerase chain reaction (PCR) was carried out on the extracted DNA, using specific primers for HHV-8. After identifying the presence of HHV-8 by amplification of its orf26 region, the orf-K1 region was amplified, sequenced and used for phylogenetic analysis. Results: From the 36 orf26-positive cases, orf-K1 was amplified and was analyzed successfully in 12 cases. Phylogenetic studies, based on the complete K1 gene/protein sequences, indicate that all strains belong to the A subtype. Specifically, six of them were related to the A1 subgroup, six to the A2 subgroup and three previously reported to the A3 subgroup. Nucleotide sequence data are reported and are available in the Genbank database under accession numbers KF829938-KF829947.

AB - Background: Kaposi's sarcoma (KS) shows a distinct geographical and ethnic distribution. Genes at both ends of the human herpesvirus 8 (HHV-8) genome have been shown to vary considerably. Seven major molecular subtypes of HHV-8 were defined based on the amino acid sequence of the open reading frame K1 (orf-K1). The aim of the present study was to characterize HHV8 isolates from hospitalized patients in Hungary. Materials and Methods: A total of 36 archival paraffin-embedded Kaposi's sarcoma tissue samples were collected. Polymerase chain reaction (PCR) was carried out on the extracted DNA, using specific primers for HHV-8. After identifying the presence of HHV-8 by amplification of its orf26 region, the orf-K1 region was amplified, sequenced and used for phylogenetic analysis. Results: From the 36 orf26-positive cases, orf-K1 was amplified and was analyzed successfully in 12 cases. Phylogenetic studies, based on the complete K1 gene/protein sequences, indicate that all strains belong to the A subtype. Specifically, six of them were related to the A1 subgroup, six to the A2 subgroup and three previously reported to the A3 subgroup. Nucleotide sequence data are reported and are available in the Genbank database under accession numbers KF829938-KF829947.

KW - HHV-8 genotypes

KW - K1

KW - Kaposi's sarcoma

KW - PCR

KW - Subtype A

UR - http://www.scopus.com/inward/record.url?scp=84897113983&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84897113983&partnerID=8YFLogxK

M3 - Article

VL - 34

SP - 893

EP - 898

JO - Anticancer Research

JF - Anticancer Research

SN - 0250-7005

IS - 2

ER -