Molecular tissue culture processing (MTCP)

A molecular and physiological method to optimize in vitro plant regeneration systems in recalcitrant species

O. Toldi, K. Ahanen, S. Sorvari, P. Scott, M. Truesdale, J. Dettmer

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

The purpose of our study was to investigate how plant growth regulators influence metabolism and development in plant tissue cultures, in order to develop a methodology for forecasting and optimising in vitro morphogenetic processes. The responsive starting explants, as well as the composition of basic compounds and growth regulators of tissue culture media were established through integrated monitoring of methylcytosine/cytosine ratios, efficiencies of photosynthesis, rates of respiration and concentrations of chlorophyll in the plant explants. We call this methodology Molecular Tissue Culture Processing (MTCP).

Original languageEnglish
Title of host publicationActa Horticulturae
Pages365-368
Number of pages4
Volume560
Publication statusPublished - 2001

Publication series

NameActa Horticulturae
Volume560
ISSN (Print)05677572

Fingerprint

tissue culture
explants
cytosine
plant growth substances
growth regulators
plant tissues
culture media
methodology
photosynthesis
chlorophyll
metabolism
monitoring
recalcitrant species

Keywords

  • Aerobic respiration
  • Carrot
  • DNA methylation
  • Metabolism
  • Photosynthesis
  • Tissue culture
  • Tobacco

ASJC Scopus subject areas

  • Horticulture

Cite this

Toldi, O., Ahanen, K., Sorvari, S., Scott, P., Truesdale, M., & Dettmer, J. (2001). Molecular tissue culture processing (MTCP): A molecular and physiological method to optimize in vitro plant regeneration systems in recalcitrant species. In Acta Horticulturae (Vol. 560, pp. 365-368). (Acta Horticulturae; Vol. 560).

Molecular tissue culture processing (MTCP) : A molecular and physiological method to optimize in vitro plant regeneration systems in recalcitrant species. / Toldi, O.; Ahanen, K.; Sorvari, S.; Scott, P.; Truesdale, M.; Dettmer, J.

Acta Horticulturae. Vol. 560 2001. p. 365-368 (Acta Horticulturae; Vol. 560).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Toldi, O, Ahanen, K, Sorvari, S, Scott, P, Truesdale, M & Dettmer, J 2001, Molecular tissue culture processing (MTCP): A molecular and physiological method to optimize in vitro plant regeneration systems in recalcitrant species. in Acta Horticulturae. vol. 560, Acta Horticulturae, vol. 560, pp. 365-368.
Toldi O, Ahanen K, Sorvari S, Scott P, Truesdale M, Dettmer J. Molecular tissue culture processing (MTCP): A molecular and physiological method to optimize in vitro plant regeneration systems in recalcitrant species. In Acta Horticulturae. Vol. 560. 2001. p. 365-368. (Acta Horticulturae).
Toldi, O. ; Ahanen, K. ; Sorvari, S. ; Scott, P. ; Truesdale, M. ; Dettmer, J. / Molecular tissue culture processing (MTCP) : A molecular and physiological method to optimize in vitro plant regeneration systems in recalcitrant species. Acta Horticulturae. Vol. 560 2001. pp. 365-368 (Acta Horticulturae).
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