Molecular theory of size exclusion chromatography for wide pore size distributions

Annamária Sepsey, Ivett Bacskay, A. Felinger

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Chromatographic processes can conveniently be modeled at a microscopic level using the molecular theory of chromatography. This molecular or microscopic theory is completely general; therefore it can be used for any chromatographic process such as adsorption, partition, ion-exchange or size exclusion chromatography. The molecular theory of chromatography allows taking into account the kinetics of the pore ingress and egress processes, the heterogeneity of the pore sizes and polymer polydispersion. In this work, we assume that the pore size in the stationary phase of chromatographic columns is governed by a wide lognormal distribution. This property is integrated into the molecular model of size exclusion chromatography and the moments of the elution profiles were calculated for several kinds of pore structure. Our results demonstrate that wide pore size distributions have strong influence on the retention properties (retention time, peak width, and peak shape) of macromolecules. The novel model allows us to estimate the real pore size distribution of commonly used HPLC stationary phases, and the effect of this distribution on the size exclusion process.

Original languageEnglish
Pages (from-to)52-60
Number of pages9
JournalJournal of Chromatography A
Volume1331
DOIs
Publication statusPublished - Feb 28 2014

Fingerprint

Size exclusion chromatography
Pore size
Gel Chromatography
Chromatography
Molecular Models
Ion Exchange
Adsorption
Polymers
High Pressure Liquid Chromatography
Pore structure
Macromolecules
Ion exchange
Kinetics

Keywords

  • Pore size distribution
  • Size exclusion chromatography
  • Stochastic theory

ASJC Scopus subject areas

  • Analytical Chemistry
  • Organic Chemistry
  • Biochemistry

Cite this

Molecular theory of size exclusion chromatography for wide pore size distributions. / Sepsey, Annamária; Bacskay, Ivett; Felinger, A.

In: Journal of Chromatography A, Vol. 1331, 28.02.2014, p. 52-60.

Research output: Contribution to journalArticle

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