Molekuláris módszerek: Új lehetoségek az adenovíruskutatás tükrében

Translated title of the contribution: Molecular techniques: New opportunities in the light of adenovirus research

Research output: Contribution to journalArticle

Abstract

The author, through presenting their latest achievements obtained in the field of adenovirus research, summarizes the new possibilities residing in modern molecular techniques that have revolutionized the methods of virus research and diagnostics. Among the novel methods, the polymerase chain reaction (PCR) has the greatest significance. Because of the large and ever growing number of known virus serotypes, classical serological identification of new isolates has become exceptionally cumbersome nowadays. Moreover, reliable reference strain and serum collections are rarely available in the laboratories. PCR combined with sequencing provides a fast and efficient alternative. The new viruses identified and classified by such methods are called types instead of serotypes. In different veterinary laboratories, numerous adenovirus strains that have been isolated in different veterinary laboratories in the past couple of decades, are yet uncharacterized. Now these viruses can be typed and classified by the sequence analysis of certain genes. The other great advantage of the method is that it allows the molecular characterization of non-isolated viruses. This is especially valuable for the study of the adenoviruses of wild living animals when isolation of the virus is usually not a realistic option. The research group of the author was the first in the world to determine the complete genomic sequence of an adenovirus exclusively by PCRs from infected organ samples of diseased and dead birds without successful isolation of the virus. Organ samples from different vertebrate animals found dead in zoos, pet shops or in the wild are being screened by PCR continuously in the author's laboratory. Faecal samples are equally suitable for screening purposes. Besides birds and reptiles, high adenoviral infection rate was demonstrated recently among domestic bats.

Original languageHungarian
Pages (from-to)498-505
Number of pages8
JournalMagyar Allatorvosok Lapja
Volume133
Issue number8
Publication statusPublished - Aug 1 2011

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ASJC Scopus subject areas

  • veterinary(all)

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