Molecular standardization of mating type terminology in the Gibberella fujikuroi species complex

Z. Kerényi, K. Zeller, L. Hornok, J. F. Leslie

Research output: Contribution to journalArticle

126 Citations (Scopus)

Abstract

Mating type in the Gibberella fujikuroi species complex is controlled by a single locus with two alleles and is usually identified following sexual crosses with standard, female-fertile tester isolates. The mating type alleles have been arbitrarily designated '+' and '-' within each biological species, and the nomenclature is tied to the standard tester strains. We developed a pair of PCR primers that can be used to amplify a unique fragment of one of the mating type alleles (MAT-2) from at least seven of the biological species in this species complex. Based on the amplification pattern, we propose a replacement for the existing, arbitrary +/- terminology that is presently in use. The new terminology is based on DNA sequence similarities between the mating type allele fragments from the biological species of the G. fujikuroi species complex and the corresponding fragments from other filamentous ascomycetes.

Original languageEnglish
Pages (from-to)4071-4076
Number of pages6
JournalApplied and environmental microbiology
Volume65
Issue number9
DOIs
Publication statusPublished - Sep 1999

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

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