Molecular mechanisms of developmentally programmed crinophagy in Drosophila

Tamás Csizmadia, Péter Lorincz, Krisztina Hegedus, Szilvia Széplaki, Péter Low, G. Juhász

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

At the onset of metamorphosis, Drosophila salivary gland cells undergo a burst of glue granule secretion to attach the forming pupa to a solid surface. Here, we show that excess granules evading exocytosis are degraded via direct fusion with lysosomes, a secretory granule-specific autophagic process known as crinophagy. We find that the tethering complex HOPS (homotypic fusion and protein sorting); the small GTPases Rab2, Rab7, and its effector, PLE KHM1; and a SNAP receptor complex consisting of Syntaxin 13, Snap29, and Vamp7 are all required for the fusion of secretory granules with lysosomes. Proper glue degradation within lysosomes also requires the Uvrag-containing Vps34 lipid kinase complex and the v-ATPase proton pump, whereas Atg genes involved in macroautophagy are dispensable for crinophagy. Our work establishes the molecular mechanism of developmentally programmed crinophagy in Drosophila and paves the way for analyzing this process in metazoans.

Original languageEnglish
Pages (from-to)361-374
Number of pages14
JournalJournal of Cell Biology
Volume217
Issue number1
DOIs
Publication statusPublished - Jan 1 2018

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Lysosomes
Drosophila
Secretory Vesicles
Adhesives
Qa-SNARE Proteins
SNARE Proteins
Proton Pumps
Pupa
Monomeric GTP-Binding Proteins
Autophagy
Exocytosis
Protein Transport
Salivary Glands
Adenosine Triphosphatases
Phosphotransferases
Lipids
Genes

ASJC Scopus subject areas

  • Cell Biology

Cite this

Molecular mechanisms of developmentally programmed crinophagy in Drosophila. / Csizmadia, Tamás; Lorincz, Péter; Hegedus, Krisztina; Széplaki, Szilvia; Low, Péter; Juhász, G.

In: Journal of Cell Biology, Vol. 217, No. 1, 01.01.2018, p. 361-374.

Research output: Contribution to journalArticle

Csizmadia, T, Lorincz, P, Hegedus, K, Széplaki, S, Low, P & Juhász, G 2018, 'Molecular mechanisms of developmentally programmed crinophagy in Drosophila', Journal of Cell Biology, vol. 217, no. 1, pp. 361-374. https://doi.org/10.1083/jcb.201702145
Csizmadia, Tamás ; Lorincz, Péter ; Hegedus, Krisztina ; Széplaki, Szilvia ; Low, Péter ; Juhász, G. / Molecular mechanisms of developmentally programmed crinophagy in Drosophila. In: Journal of Cell Biology. 2018 ; Vol. 217, No. 1. pp. 361-374.
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