Molecular investigations of the bat tick Argas vespertilionis (Ixodida: Argasidae) and Babesia vesperuginis (Apicomplexa: Piroplasmida) reflect “bat connection” between Central Europe and Central Asia

Sándor Hornok, Krisztina Szőke, Tamás Görföl, G. Földvári, Vuong Tan Tu, Nóra Takács, Jenő Kontschán, Attila D. Sándor, Péter Estók, Sara Epis, Sándor Boldogh, Dávid Kováts, Yuanzhi Wang

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Argas vespertilionis is a geographically widespread haematophagous ectoparasite species of bats in the Old World, with a suspected role in the transmission of Babesia vesperuginis. The aims of the present study were (1) to molecularly screen A. vespertilionis larvae (collected in Europe, Africa and Asia) for the presence of piroplasms, and (2) to analyze mitochondrial markers of A. vespertilionis larvae from Central Asia (Xinjiang Province, Northwestern China) in a phylogeographical context. Out of the 193 DNA extracts from 321 A. vespertilionis larvae, 12 contained piroplasm DNA (10 from Hungary, two from China). Sequencing showed the exclusive presence of B. vesperuginis, with 100% sequence identity between samples from Hungary and China. In addition, A. vespertilionis cytochrome oxidase c subunit 1 (cox1) and 16S rRNA gene sequences had 99.1–99.2 and 99.5–100% similarities, respectively, between Hungary and China. Accordingly, in the phylogenetic analyses A. vespertilionis from China clustered with haplotypes from Europe, and (with high support) outside the group formed by haplotypes from Southeast Asia. This is the first molecular evidence on the occurrence of B. vesperuginis in Asia. Bat ticks from hosts in Vespertilionidae contained only the DNA of B. vesperuginis (in contrast with what was reported on bat ticks from Rhinolophidae and Miniopteridae). Molecular taxonomic analyses of A. vespertilionis and B. vesperuginis suggest a genetic link of bat parasites between Central Europe and Central Asia, which is epidemiologically relevant in the context of any pathogens associated with bats.

Original languageEnglish
Pages (from-to)69-77
Number of pages9
JournalExperimental and Applied Acarology
Volume72
Issue number1
DOIs
Publication statusPublished - May 1 2017

Fingerprint

Ixodida
Argas
Piroplasmida
Argasidae
Babesia
tick
Central Asia
bat
Central European region
Chiroptera
ticks
China
Hungary
larva
DNA
larvae
haplotypes
Rhinolophidae
ectoparasite
Vespertilionidae

Keywords

  • Argas
  • Babesia
  • Chiroptera
  • Phylogeography
  • Soft tick

ASJC Scopus subject areas

  • Ecology
  • Insect Science

Cite this

Molecular investigations of the bat tick Argas vespertilionis (Ixodida : Argasidae) and Babesia vesperuginis (Apicomplexa: Piroplasmida) reflect “bat connection” between Central Europe and Central Asia. / Hornok, Sándor; Szőke, Krisztina; Görföl, Tamás; Földvári, G.; Tu, Vuong Tan; Takács, Nóra; Kontschán, Jenő; Sándor, Attila D.; Estók, Péter; Epis, Sara; Boldogh, Sándor; Kováts, Dávid; Wang, Yuanzhi.

In: Experimental and Applied Acarology, Vol. 72, No. 1, 01.05.2017, p. 69-77.

Research output: Contribution to journalArticle

Hornok, Sándor ; Szőke, Krisztina ; Görföl, Tamás ; Földvári, G. ; Tu, Vuong Tan ; Takács, Nóra ; Kontschán, Jenő ; Sándor, Attila D. ; Estók, Péter ; Epis, Sara ; Boldogh, Sándor ; Kováts, Dávid ; Wang, Yuanzhi. / Molecular investigations of the bat tick Argas vespertilionis (Ixodida : Argasidae) and Babesia vesperuginis (Apicomplexa: Piroplasmida) reflect “bat connection” between Central Europe and Central Asia. In: Experimental and Applied Acarology. 2017 ; Vol. 72, No. 1. pp. 69-77.
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AU - Görföl, Tamás

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