Molecular interaction of a new antibacterial polymer with a supported lipid bilayer measured by an in situ label-free optical technique

R. Horváth, Balázs Kobzi, Helmut Keul, Martin Moeller, E. Kiss

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The interaction of the antibacterial polymer-branched poly(ethylene imine) substituted with quaternary ammonium groups, PEO and alkyl chains, PEI25QI5J5A815-with a solid supported lipid bilayer was investigated using surface sensitive optical waveguide spectroscopy. The analysis of the optogeometrical parameters was extended developing a new composite layer model in which the structural and optical anisotropy of the molecular layers was taken into consideration. Following in situ the change of optical birefringence we were able to determine the composition of the lipid/polymer surface layer as well as the displacement of lipid bilayer by the antibacterial polymer without using additional labeling. Comparative assessment of the data of layer thickness and optical anisotropy helps to reveal the molecular mechanism of antibacterial effect of the polymer investigated.

Original languageEnglish
Pages (from-to)9722-9736
Number of pages15
JournalInternational Journal of Molecular Sciences
Volume14
Issue number5
DOIs
Publication statusPublished - May 2013

Fingerprint

Lipid bilayers
Molecular interactions
Lipid Bilayers
molecular interactions
lipids
Labels
Polymers
Optical anisotropy
Anisotropy
polymers
Birefringence
anisotropy
Optical waveguides
Polyethylene oxides
Ammonium Compounds
optical waveguides
Labeling
Lipids
imines
marking

Keywords

  • Antibacterial polymer
  • Label-free measurement
  • Optical anisotropy
  • Optical waveguide spectroscopy
  • Supported lipid bilayer

ASJC Scopus subject areas

  • Computer Science Applications
  • Molecular Biology
  • Catalysis
  • Inorganic Chemistry
  • Spectroscopy
  • Organic Chemistry
  • Physical and Theoretical Chemistry

Cite this

Molecular interaction of a new antibacterial polymer with a supported lipid bilayer measured by an in situ label-free optical technique. / Horváth, R.; Kobzi, Balázs; Keul, Helmut; Moeller, Martin; Kiss, E.

In: International Journal of Molecular Sciences, Vol. 14, No. 5, 05.2013, p. 9722-9736.

Research output: Contribution to journalArticle

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