Molecular identification of Trichoderma species from Russia, Siberia and the Himalaya

C. Kullnig, G. Szakács, C. P. Kubicek

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

About 35 Trichoderma species are currently recognised on the basis of morphological and molecular characters. Besides the role of a few of these species in biotechnology, several seem to play prominent roles in soil ecosystems. With a goal of investigating global biodiversity in Trichoderma, we report on the occurrence of Trichoderma spp. in Russia (Moscow and Ural areas), Siberia (Krasnoyarsk area) and the Himalayan mountains - areas from which no Trichoderma isolates are so far available. The ITS 1 and 2 sequence of the rDNA cluster of the 75 isolates obtained was compared with that of ex-type strains and taxonomically established isolates of Trichoderma. Thirty-nine isolates were positively identified as T. atroviride, T. virens, T. hamatum, T. asperellum, T. koningii and T. oblongisporum. A further 26 isolates yielded six closely related ITS1/2 sequence types, which are highly similar yet different from the ex-(neo)type strains of T. harzianum and T. inhamatum. Some of these genotypes (i.e. 1 and 2a) occurred only in Russia/Siberia, whereas others (2b, 3, 4 and 5) were found only in the Himalayas. RAPD analysis was consistent with these genotypes, and revealed genetic homogeneity even between strains from widely separated areas. Parsimony analysis placed these five genotypes, together with T. harzianum, T. inhamatum and the mushroom-aggressive T. harzianum 'biotype 2' in a large, unresolved 'harzianum' clade. Ten isolates were not safely alignable within known species, and five of them may be undescribed taxa: one isolate from 2700 m elevation in the Himalayas, which clustered in parsimony analysis at a basal position in section Longibrachiatum; and four isolates, displaying two closely related sequence types, forming a separate clade with T. stromaticum. The five remaining isolates also exhibited three unique ITS1 and 2 sequence patterns, but parsimony analysis placed them into the unresolved 'harzianum' clade, and their relationship to T. harzianum is thus unclear. The study shows that molecular screening of uninvestigated geographic areas can lead to the identification of isolates with new ITS1 and ITS2 sequence patterns, some of which may be new taxa. It also reveals that T. harzianum is at present the genetically most diverse member of the genus.

Original languageEnglish
Pages (from-to)1117-1125
Number of pages9
JournalMycological Research
Volume104
Issue number9
DOIs
Publication statusPublished - 2000

Fingerprint

Siberia
Trichoderma
parsimony analysis
Russia
genotype
Genotype
Trichoderma oblongisporum
biotype
soil ecosystem
mushroom
biotechnology
new taxon
homogeneity
Trichoderma asperellum
Trichoderma atroviride
Trichoderma hamatum
Trichoderma koningii
Trichoderma virens
Moscow
biodiversity

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Plant Science

Cite this

Molecular identification of Trichoderma species from Russia, Siberia and the Himalaya. / Kullnig, C.; Szakács, G.; Kubicek, C. P.

In: Mycological Research, Vol. 104, No. 9, 2000, p. 1117-1125.

Research output: Contribution to journalArticle

@article{437421411a9041b187f7d3c44926107d,
title = "Molecular identification of Trichoderma species from Russia, Siberia and the Himalaya",
abstract = "About 35 Trichoderma species are currently recognised on the basis of morphological and molecular characters. Besides the role of a few of these species in biotechnology, several seem to play prominent roles in soil ecosystems. With a goal of investigating global biodiversity in Trichoderma, we report on the occurrence of Trichoderma spp. in Russia (Moscow and Ural areas), Siberia (Krasnoyarsk area) and the Himalayan mountains - areas from which no Trichoderma isolates are so far available. The ITS 1 and 2 sequence of the rDNA cluster of the 75 isolates obtained was compared with that of ex-type strains and taxonomically established isolates of Trichoderma. Thirty-nine isolates were positively identified as T. atroviride, T. virens, T. hamatum, T. asperellum, T. koningii and T. oblongisporum. A further 26 isolates yielded six closely related ITS1/2 sequence types, which are highly similar yet different from the ex-(neo)type strains of T. harzianum and T. inhamatum. Some of these genotypes (i.e. 1 and 2a) occurred only in Russia/Siberia, whereas others (2b, 3, 4 and 5) were found only in the Himalayas. RAPD analysis was consistent with these genotypes, and revealed genetic homogeneity even between strains from widely separated areas. Parsimony analysis placed these five genotypes, together with T. harzianum, T. inhamatum and the mushroom-aggressive T. harzianum 'biotype 2' in a large, unresolved 'harzianum' clade. Ten isolates were not safely alignable within known species, and five of them may be undescribed taxa: one isolate from 2700 m elevation in the Himalayas, which clustered in parsimony analysis at a basal position in section Longibrachiatum; and four isolates, displaying two closely related sequence types, forming a separate clade with T. stromaticum. The five remaining isolates also exhibited three unique ITS1 and 2 sequence patterns, but parsimony analysis placed them into the unresolved 'harzianum' clade, and their relationship to T. harzianum is thus unclear. The study shows that molecular screening of uninvestigated geographic areas can lead to the identification of isolates with new ITS1 and ITS2 sequence patterns, some of which may be new taxa. It also reveals that T. harzianum is at present the genetically most diverse member of the genus.",
author = "C. Kullnig and G. Szak{\'a}cs and Kubicek, {C. P.}",
year = "2000",
doi = "10.1017/S0953756200002604",
language = "English",
volume = "104",
pages = "1117--1125",
journal = "Fungal Biology",
issn = "1878-6146",
publisher = "Elsevier",
number = "9",

}

TY - JOUR

T1 - Molecular identification of Trichoderma species from Russia, Siberia and the Himalaya

AU - Kullnig, C.

AU - Szakács, G.

AU - Kubicek, C. P.

PY - 2000

Y1 - 2000

N2 - About 35 Trichoderma species are currently recognised on the basis of morphological and molecular characters. Besides the role of a few of these species in biotechnology, several seem to play prominent roles in soil ecosystems. With a goal of investigating global biodiversity in Trichoderma, we report on the occurrence of Trichoderma spp. in Russia (Moscow and Ural areas), Siberia (Krasnoyarsk area) and the Himalayan mountains - areas from which no Trichoderma isolates are so far available. The ITS 1 and 2 sequence of the rDNA cluster of the 75 isolates obtained was compared with that of ex-type strains and taxonomically established isolates of Trichoderma. Thirty-nine isolates were positively identified as T. atroviride, T. virens, T. hamatum, T. asperellum, T. koningii and T. oblongisporum. A further 26 isolates yielded six closely related ITS1/2 sequence types, which are highly similar yet different from the ex-(neo)type strains of T. harzianum and T. inhamatum. Some of these genotypes (i.e. 1 and 2a) occurred only in Russia/Siberia, whereas others (2b, 3, 4 and 5) were found only in the Himalayas. RAPD analysis was consistent with these genotypes, and revealed genetic homogeneity even between strains from widely separated areas. Parsimony analysis placed these five genotypes, together with T. harzianum, T. inhamatum and the mushroom-aggressive T. harzianum 'biotype 2' in a large, unresolved 'harzianum' clade. Ten isolates were not safely alignable within known species, and five of them may be undescribed taxa: one isolate from 2700 m elevation in the Himalayas, which clustered in parsimony analysis at a basal position in section Longibrachiatum; and four isolates, displaying two closely related sequence types, forming a separate clade with T. stromaticum. The five remaining isolates also exhibited three unique ITS1 and 2 sequence patterns, but parsimony analysis placed them into the unresolved 'harzianum' clade, and their relationship to T. harzianum is thus unclear. The study shows that molecular screening of uninvestigated geographic areas can lead to the identification of isolates with new ITS1 and ITS2 sequence patterns, some of which may be new taxa. It also reveals that T. harzianum is at present the genetically most diverse member of the genus.

AB - About 35 Trichoderma species are currently recognised on the basis of morphological and molecular characters. Besides the role of a few of these species in biotechnology, several seem to play prominent roles in soil ecosystems. With a goal of investigating global biodiversity in Trichoderma, we report on the occurrence of Trichoderma spp. in Russia (Moscow and Ural areas), Siberia (Krasnoyarsk area) and the Himalayan mountains - areas from which no Trichoderma isolates are so far available. The ITS 1 and 2 sequence of the rDNA cluster of the 75 isolates obtained was compared with that of ex-type strains and taxonomically established isolates of Trichoderma. Thirty-nine isolates were positively identified as T. atroviride, T. virens, T. hamatum, T. asperellum, T. koningii and T. oblongisporum. A further 26 isolates yielded six closely related ITS1/2 sequence types, which are highly similar yet different from the ex-(neo)type strains of T. harzianum and T. inhamatum. Some of these genotypes (i.e. 1 and 2a) occurred only in Russia/Siberia, whereas others (2b, 3, 4 and 5) were found only in the Himalayas. RAPD analysis was consistent with these genotypes, and revealed genetic homogeneity even between strains from widely separated areas. Parsimony analysis placed these five genotypes, together with T. harzianum, T. inhamatum and the mushroom-aggressive T. harzianum 'biotype 2' in a large, unresolved 'harzianum' clade. Ten isolates were not safely alignable within known species, and five of them may be undescribed taxa: one isolate from 2700 m elevation in the Himalayas, which clustered in parsimony analysis at a basal position in section Longibrachiatum; and four isolates, displaying two closely related sequence types, forming a separate clade with T. stromaticum. The five remaining isolates also exhibited three unique ITS1 and 2 sequence patterns, but parsimony analysis placed them into the unresolved 'harzianum' clade, and their relationship to T. harzianum is thus unclear. The study shows that molecular screening of uninvestigated geographic areas can lead to the identification of isolates with new ITS1 and ITS2 sequence patterns, some of which may be new taxa. It also reveals that T. harzianum is at present the genetically most diverse member of the genus.

UR - http://www.scopus.com/inward/record.url?scp=0033752847&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033752847&partnerID=8YFLogxK

U2 - 10.1017/S0953756200002604

DO - 10.1017/S0953756200002604

M3 - Article

VL - 104

SP - 1117

EP - 1125

JO - Fungal Biology

JF - Fungal Biology

SN - 1878-6146

IS - 9

ER -