Molecular identification of Anaplasma marginale and rickettsial endosymbionts in blood-sucking flies (Diptera: Tabanidae, Muscidae) and hard ticks (Acari: Ixodidae)

S. Hornok, G. Földvári, Vilmos Elek, Victoria Naranjo, R. Farkas, José de la Fuente

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

In an attempt to identify the main vector and possible transmission routes of Anaplasma spp. in a region of Hungary with high prevalence of ovine and bovine anaplasmosis, DNA was extracted from 316 haematophagous arthropods (individually or in pools), including 4 species of ixodid ticks, 6 species of tabanid flies and hornflies. Midichloria-like organisms were identified with PCR (amplifying a portion of the 16S rRNA gene) and sequencing from Dermacentor marginatus and Ixodes ricinus. Significantly higher 16S positive D. marginatus individuals were collected in March than in April, suggesting earlier questing of ticks that contain rickettsial agents (thus endosymbionts). Midichloria- and Wolbachia-like organisms were also found in randomly caught horse flies (Tabanus bovinus and T. tergestinus) as well as hornflies (Haematobia irritans), respectively, with 97-99% similarity to sequences deposited in the GenBank. Although all ticks were negative in the Anaplasma spp.-specific msp4 PCR, four individuals of T. bovinus collected near to grazing cattle were positive for Anaplasma marginale. The results of the present study provide the first molecular evidence for the potential mechanical vector role of T. bovinus in the transmission of A. marginale, and broaden the range of haematophagous arthropods harbouring Midichloria-like bacteria, for the first time in any Dermacentor or Tabanus species.

Original languageEnglish
Pages (from-to)354-359
Number of pages6
JournalVeterinary Parasitology
Volume154
Issue number3-4
DOIs
Publication statusPublished - Jul 4 2008

Fingerprint

Anaplasma marginale
Muscidae
hematophagy
Ixodidae
Tabanidae
endosymbionts
Ticks
Anaplasma
hematophagous arthropods
Dermacentor marginatus
Tabanus
Diptera
Dermacentor
ticks
Acari
Arthropods
bovine anaplasmosis
Anaplasmosis
Wolbachia
Haematobia irritans

Keywords

  • Anaplasma marginale
  • Dermacentor
  • Horn fly
  • Horse fly
  • Midichloria
  • Tabanus
  • Wolbachia

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Parasitology
  • veterinary(all)

Cite this

Molecular identification of Anaplasma marginale and rickettsial endosymbionts in blood-sucking flies (Diptera : Tabanidae, Muscidae) and hard ticks (Acari: Ixodidae). / Hornok, S.; Földvári, G.; Elek, Vilmos; Naranjo, Victoria; Farkas, R.; de la Fuente, José.

In: Veterinary Parasitology, Vol. 154, No. 3-4, 04.07.2008, p. 354-359.

Research output: Contribution to journalArticle

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