Molecular identification of a novel hantavirus in Malaysian bronze tube-nosed bats (murina aenea)

Brigitta Zana, Gábor Kemenesi, Dóra Buzás, Gábor Csorba, Tamás Görföl, Faisal Ali Anwarali Khan, Nurul Farah Diyana Ahmad Tahir, Safia Zeghbib, Mónika Madai, Henrietta Papp, Fanni Földes, Péter Urbán, Róbert Herczeg, Gábor Endre Tóth, Ferenc Jakab

Research output: Contribution to journalArticle

Abstract

In the past ten years, several novel hantaviruses were discovered in shrews, moles, and bats, suggesting the dispersal of hantaviruses in many animal taxa other than rodents during their evolution. Interestingly, the coevolutionary analyses of most recent studies have raised the possibility that nonrodents may have served as the primordial mammalian host and harboured the ancestors of rodent-borne hantaviruses as well. The aim of our study was to investigate the presence of hantaviruses in bat lung tissue homogenates originally collected for taxonomic purposes in Malaysia in 2015. Hantavirus-specific nested RT-PCR screening of 116 samples targeting the L segment of the virus has revealed the positivity of two lung tissue homogenates originating from two individuals, a female and a male of the Murina aenea bat species collected at the same site and sampling occasion. Nanopore sequencing of hantavirus positive samples resulted in partial genomic data from S, M, and L genome segments. The obtained results indicate molecular evidence for hantaviruses in the M. aenea bat species. Sequence analysis of the PCR amplicon and partial genome segments suggests that the identified virus may represent a novel species in the Mobatvirus genus within the Hantaviridae family. Our results provide additional genomic data to help extend our knowledge about the evolution of these viruses.

Original languageEnglish
Article number887
JournalViruses
Volume11
Issue number10
DOIs
Publication statusPublished - Sep 21 2019

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Keywords

  • MinION
  • Mobatvirus
  • One health concept
  • Sarawak mobatvirus
  • Tb1-Lu

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology

Cite this

Zana, B., Kemenesi, G., Buzás, D., Csorba, G., Görföl, T., Khan, F. A. A., Tahir, N. F. D. A., Zeghbib, S., Madai, M., Papp, H., Földes, F., Urbán, P., Herczeg, R., Tóth, G. E., & Jakab, F. (2019). Molecular identification of a novel hantavirus in Malaysian bronze tube-nosed bats (murina aenea). Viruses, 11(10), [887]. https://doi.org/10.3390/v11100887