Molecular hydrogen alleviates asphyxia-induced neuronal cyclooxygenase-2 expression in newborn pigs

Viktória Varga, J. Németh, Orsolya Oláh, Valéria Tóth-Szűki, Viktória Kovács, Gábor Remzső, F. Domoki

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Cyclooxygenase-2 (COX-2) has an established role in the pathogenesis of hypoxic-ischemic encephalopathy (HIE). In this study we sought to determine whether COX-2 was induced by asphyxia in newborn pigs, and whether neuronal COX-2 levels were affected by H2 treatment. Piglets were subjected to either 8 min of asphyxia or a more severe 20 min of asphyxia followed by H2 treatment (inhaling room air containing 2.1% H2 for 4 h). COX-2 immunohistochemistry was performed on brain samples from surviving piglets 24 h after asphyxia. The percentages of COX-2-immunopositive neurons were determined in cortical and subcortical areas. Only in piglets with more severe HIE, we observed significant, region-specific increases in neuronal COX-2 expression within the parietal and occipital cortices and in the CA3 hippocampal subfield. H2 treatment essentially prevented the increases in COX-2-immunopositive neurons. In the parietal cortex, the attenuation of COX-2 induction was associated with reduced 8'-hydroxy-2′-deoxyguanozine immunoreactivity and retained microglial ramifcation index, which are markers of oxidative stress and neuroinfiammation, respectively. This study demonstrates for the first time that asphyxia elevates neuronal COX-2 expression in a piglet HIE model. Neuronal COX-2 induction may play region-specific roles in brain lesion progression during HIE development, and inhibition of this response may contribute to the antioxidant/anti-infiammatory neuroprotective effects of H2 treatment.

Original languageEnglish
Pages (from-to)1273-1283
Number of pages11
JournalActa Pharmacologica Sinica
Volume39
Issue number8
DOIs
Publication statusPublished - Aug 1 2018

Fingerprint

Asphyxia
Cyclooxygenase 2
Hydrogen
Swine
Brain Hypoxia-Ischemia
Parietal Lobe
Neurons
Occipital Lobe
Brain
Neuroprotective Agents
Therapeutics
Inhalation
Oxidative Stress
Antioxidants
Immunohistochemistry
Air

Keywords

  • 8′-hydroxy-2′-deoxyguanozine
  • COX-2
  • hypoxic-ischemic encephalopathy
  • microglia
  • molecular hydrogen
  • neuroprotection
  • newborn pigs

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)

Cite this

Molecular hydrogen alleviates asphyxia-induced neuronal cyclooxygenase-2 expression in newborn pigs. / Varga, Viktória; Németh, J.; Oláh, Orsolya; Tóth-Szűki, Valéria; Kovács, Viktória; Remzső, Gábor; Domoki, F.

In: Acta Pharmacologica Sinica, Vol. 39, No. 8, 01.08.2018, p. 1273-1283.

Research output: Contribution to journalArticle

Varga, Viktória ; Németh, J. ; Oláh, Orsolya ; Tóth-Szűki, Valéria ; Kovács, Viktória ; Remzső, Gábor ; Domoki, F. / Molecular hydrogen alleviates asphyxia-induced neuronal cyclooxygenase-2 expression in newborn pigs. In: Acta Pharmacologica Sinica. 2018 ; Vol. 39, No. 8. pp. 1273-1283.
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