Hagyományos és DH (doubled haploid) búzafajták, valamint DH vonalaik homogenitásának molekuláris elemzése

Translated title of the contribution: Molecular homogeneity of conventional and doubled haploid wheat cultivars and their DH lines

O. Törjék, E. Kiss, Katalin Mázikné Tokei, György Hutvágner, D. Silhavy, Z. Bánfalvi, Zoltán Kertész, János Pauk, L. Heszky

Research output: Contribution to journalArticle

Abstract

A traditional cultivar (GK Góbé), a cultivar of doubled haploid (DH) origin (GK Délibáb), various DH lines of GK Góbé (first cycle DH lines) and DH lines of GK Délibáb (second cycle DH lines) were compared with PCR-bascd molecular techniques. High molecular weight DNA was isolated from randomly selected individuals of these groups and analysed by the RAPD, SSR, STS and AFLP methods. The objective of the analyses was to determine the existence or magnitude of the difference between cultivars produced by classical and haploid methods (populations are represented by the individuals of each group), and to find which marker system would be most suitable to investigate the homogeneity of DH populations. From the 30 RAPD primers tested, only 6 differentiated the two cultivars (GK Góbé and Délibáb). Individual polymorphism could not be observed. There were 8 fragments generated on 15 loci with 12 SSR and STS primers, which were suitable to differentiate the two cultivars. Individual polymorphisms could be detected within the cultivar GK Délibáb (cultivar of doubled haploid origin) with primer WMS186. In the AFLP analyses, 7 of 8 primer combinations were suitable to show differences, resulting in an average of 100-150 fragments. Eighty-one polymorphic fragments were obtained with these 7 primer combinations. Twenty-three of the 81 polymorphic bands (markers) could detect individual differences. Nine of them were suitable to distingiushc cultivar GK Góbé and its DH group. Based on AFLP fragments, the fewest individual polymorphisms were obtained within the DH group of GK Délibáb (second cycle DH lines). The genetic stability of doubled haploid lines of androgenic origin is the prerequisite of their breeding value. The productivity and adaptability of the new DH varieties were similar to the conventional cultivars in field trials. In the present investigation conventional and DH varieties were analysed and compared by different molecular (RAPD, SSR, STS and AFL) methods. No significant molecular differences were observed between these two groups, including the individual polymorphism within a group. Based on the results it could be concluded that the level of homozygosity of conventional wheat varieties is probably as high as it is in DH cultivars. Further molecular analyses arc necessary to confirm this conclusion. It can be assumed that the adaptability of conventional and DH varieties originated mainly from the epigenetic and not the genetic variability of these populations.

Original languageHungarian
Pages (from-to)7-20
Number of pages14
JournalNovenytermeles
Volume51
Issue number1
Publication statusPublished - Feb 2002

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doubled haploids
wheat
cultivars
genetic polymorphism
amplified fragment length polymorphism
genetic stability
homozygosity
breeding value
methodology
epigenetics
haploidy
field experimentation

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

Hagyományos és DH (doubled haploid) búzafajták, valamint DH vonalaik homogenitásának molekuláris elemzése. / Törjék, O.; Kiss, E.; Mázikné Tokei, Katalin; Hutvágner, György; Silhavy, D.; Bánfalvi, Z.; Kertész, Zoltán; Pauk, János; Heszky, L.

In: Novenytermeles, Vol. 51, No. 1, 02.2002, p. 7-20.

Research output: Contribution to journalArticle

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title = "Hagyom{\'a}nyos {\'e}s DH (doubled haploid) b{\'u}zafajt{\'a}k, valamint DH vonalaik homogenit{\'a}s{\'a}nak molekul{\'a}ris elemz{\'e}se",
abstract = "A traditional cultivar (GK G{\'o}b{\'e}), a cultivar of doubled haploid (DH) origin (GK D{\'e}lib{\'a}b), various DH lines of GK G{\'o}b{\'e} (first cycle DH lines) and DH lines of GK D{\'e}lib{\'a}b (second cycle DH lines) were compared with PCR-bascd molecular techniques. High molecular weight DNA was isolated from randomly selected individuals of these groups and analysed by the RAPD, SSR, STS and AFLP methods. The objective of the analyses was to determine the existence or magnitude of the difference between cultivars produced by classical and haploid methods (populations are represented by the individuals of each group), and to find which marker system would be most suitable to investigate the homogeneity of DH populations. From the 30 RAPD primers tested, only 6 differentiated the two cultivars (GK G{\'o}b{\'e} and D{\'e}lib{\'a}b). Individual polymorphism could not be observed. There were 8 fragments generated on 15 loci with 12 SSR and STS primers, which were suitable to differentiate the two cultivars. Individual polymorphisms could be detected within the cultivar GK D{\'e}lib{\'a}b (cultivar of doubled haploid origin) with primer WMS186. In the AFLP analyses, 7 of 8 primer combinations were suitable to show differences, resulting in an average of 100-150 fragments. Eighty-one polymorphic fragments were obtained with these 7 primer combinations. Twenty-three of the 81 polymorphic bands (markers) could detect individual differences. Nine of them were suitable to distingiushc cultivar GK G{\'o}b{\'e} and its DH group. Based on AFLP fragments, the fewest individual polymorphisms were obtained within the DH group of GK D{\'e}lib{\'a}b (second cycle DH lines). The genetic stability of doubled haploid lines of androgenic origin is the prerequisite of their breeding value. The productivity and adaptability of the new DH varieties were similar to the conventional cultivars in field trials. In the present investigation conventional and DH varieties were analysed and compared by different molecular (RAPD, SSR, STS and AFL) methods. No significant molecular differences were observed between these two groups, including the individual polymorphism within a group. Based on the results it could be concluded that the level of homozygosity of conventional wheat varieties is probably as high as it is in DH cultivars. Further molecular analyses arc necessary to confirm this conclusion. It can be assumed that the adaptability of conventional and DH varieties originated mainly from the epigenetic and not the genetic variability of these populations.",
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T1 - Hagyományos és DH (doubled haploid) búzafajták, valamint DH vonalaik homogenitásának molekuláris elemzése

AU - Törjék, O.

AU - Kiss, E.

AU - Mázikné Tokei, Katalin

AU - Hutvágner, György

AU - Silhavy, D.

AU - Bánfalvi, Z.

AU - Kertész, Zoltán

AU - Pauk, János

AU - Heszky, L.

PY - 2002/2

Y1 - 2002/2

N2 - A traditional cultivar (GK Góbé), a cultivar of doubled haploid (DH) origin (GK Délibáb), various DH lines of GK Góbé (first cycle DH lines) and DH lines of GK Délibáb (second cycle DH lines) were compared with PCR-bascd molecular techniques. High molecular weight DNA was isolated from randomly selected individuals of these groups and analysed by the RAPD, SSR, STS and AFLP methods. The objective of the analyses was to determine the existence or magnitude of the difference between cultivars produced by classical and haploid methods (populations are represented by the individuals of each group), and to find which marker system would be most suitable to investigate the homogeneity of DH populations. From the 30 RAPD primers tested, only 6 differentiated the two cultivars (GK Góbé and Délibáb). Individual polymorphism could not be observed. There were 8 fragments generated on 15 loci with 12 SSR and STS primers, which were suitable to differentiate the two cultivars. Individual polymorphisms could be detected within the cultivar GK Délibáb (cultivar of doubled haploid origin) with primer WMS186. In the AFLP analyses, 7 of 8 primer combinations were suitable to show differences, resulting in an average of 100-150 fragments. Eighty-one polymorphic fragments were obtained with these 7 primer combinations. Twenty-three of the 81 polymorphic bands (markers) could detect individual differences. Nine of them were suitable to distingiushc cultivar GK Góbé and its DH group. Based on AFLP fragments, the fewest individual polymorphisms were obtained within the DH group of GK Délibáb (second cycle DH lines). The genetic stability of doubled haploid lines of androgenic origin is the prerequisite of their breeding value. The productivity and adaptability of the new DH varieties were similar to the conventional cultivars in field trials. In the present investigation conventional and DH varieties were analysed and compared by different molecular (RAPD, SSR, STS and AFL) methods. No significant molecular differences were observed between these two groups, including the individual polymorphism within a group. Based on the results it could be concluded that the level of homozygosity of conventional wheat varieties is probably as high as it is in DH cultivars. Further molecular analyses arc necessary to confirm this conclusion. It can be assumed that the adaptability of conventional and DH varieties originated mainly from the epigenetic and not the genetic variability of these populations.

AB - A traditional cultivar (GK Góbé), a cultivar of doubled haploid (DH) origin (GK Délibáb), various DH lines of GK Góbé (first cycle DH lines) and DH lines of GK Délibáb (second cycle DH lines) were compared with PCR-bascd molecular techniques. High molecular weight DNA was isolated from randomly selected individuals of these groups and analysed by the RAPD, SSR, STS and AFLP methods. The objective of the analyses was to determine the existence or magnitude of the difference between cultivars produced by classical and haploid methods (populations are represented by the individuals of each group), and to find which marker system would be most suitable to investigate the homogeneity of DH populations. From the 30 RAPD primers tested, only 6 differentiated the two cultivars (GK Góbé and Délibáb). Individual polymorphism could not be observed. There were 8 fragments generated on 15 loci with 12 SSR and STS primers, which were suitable to differentiate the two cultivars. Individual polymorphisms could be detected within the cultivar GK Délibáb (cultivar of doubled haploid origin) with primer WMS186. In the AFLP analyses, 7 of 8 primer combinations were suitable to show differences, resulting in an average of 100-150 fragments. Eighty-one polymorphic fragments were obtained with these 7 primer combinations. Twenty-three of the 81 polymorphic bands (markers) could detect individual differences. Nine of them were suitable to distingiushc cultivar GK Góbé and its DH group. Based on AFLP fragments, the fewest individual polymorphisms were obtained within the DH group of GK Délibáb (second cycle DH lines). The genetic stability of doubled haploid lines of androgenic origin is the prerequisite of their breeding value. The productivity and adaptability of the new DH varieties were similar to the conventional cultivars in field trials. In the present investigation conventional and DH varieties were analysed and compared by different molecular (RAPD, SSR, STS and AFL) methods. No significant molecular differences were observed between these two groups, including the individual polymorphism within a group. Based on the results it could be concluded that the level of homozygosity of conventional wheat varieties is probably as high as it is in DH cultivars. Further molecular analyses arc necessary to confirm this conclusion. It can be assumed that the adaptability of conventional and DH varieties originated mainly from the epigenetic and not the genetic variability of these populations.

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