Molecular form of human lymphocyte membrane-bound acetylcholinesterase

Emilia Bartha, Zoltán Rakonczay, P. Kása, S. Hollán, Angéla Gyévai

Research output: Contribution to journalArticle

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Abstract

The membrane-bound acetylcholinesterase (AchE) from human peripheral blood lymphocyte gives only one symmetrical peak on sucrose density gradient centrifugation in the presence of Triton X-100 detergent, with the calculated sedimentation coefficient of 6.5 S. However, this dimeric form of AchE was converted to a monomeric 3.8 S form when treated with 2-mercaptoethanol and iodoacetic acid. The results are consistent with studies which have shown by sodium dodecyl sulfate gel electrophoresis that the enzyme is built up of two identical monomers interlinked by disulfide bond(s). Under reducing conditions, revealed a single species of 70000 molecular weight, whereas under non-reducting conditions, another species of 140000 molecular weight of the AchE was found. Polyacrylamide gel electrophoresis indicated a single band with AchE activity in the presence of Triton X-100. In contrast, in the absence of the same detergent multiple band pattern could be observed. These results suggest that membrane-bound AchE enzyme is present in homogenous dimeric form on human lymphocyte membrane.

Original languageEnglish
Pages (from-to)1853-1860
Number of pages8
JournalLife Sciences
Volume41
Issue number15
DOIs
Publication statusPublished - Oct 12 1987

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Lymphocytes
Acetylcholinesterase
Membranes
Octoxynol
Electrophoresis
Detergents
Molecular Weight
Molecular weight
Iodoacetic Acid
Density Gradient Centrifugation
Centrifugation
Mercaptoethanol
Enzymes
Sedimentation
Sodium Dodecyl Sulfate
Disulfides
Sucrose
Polyacrylamide Gel Electrophoresis
Blood
Monomers

ASJC Scopus subject areas

  • Pharmacology

Cite this

Molecular form of human lymphocyte membrane-bound acetylcholinesterase. / Bartha, Emilia; Rakonczay, Zoltán; Kása, P.; Hollán, S.; Gyévai, Angéla.

In: Life Sciences, Vol. 41, No. 15, 12.10.1987, p. 1853-1860.

Research output: Contribution to journalArticle

Bartha, Emilia ; Rakonczay, Zoltán ; Kása, P. ; Hollán, S. ; Gyévai, Angéla. / Molecular form of human lymphocyte membrane-bound acetylcholinesterase. In: Life Sciences. 1987 ; Vol. 41, No. 15. pp. 1853-1860.
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