Amyloidosis is a general term describing disorders characterised by the pathologic deposition of some fibrillar protein, amyloid in tissues. Amyloid is an extracellularly deposited insoluble substance, composed of unbranched, cross β-patterned fibrils. Acute phase protein serum amyloid A is the precursor protein of AA amyloidosis, the type of amyloidosis geese suffer from. Chronic increase of SAA level is an essential but not sufficient precondition in this secondary disease resulting from a variety of infectious and non-infectious inflammatory conditions. Goose SAA can be considered as a significant signal protein of the disease. Although this disease causes considerable damages among goose stocks in Hungary, no specific tool has been available to detect and diagnose amyloidosis up to now. The present study demonstrates the cloning of the coding region and expression of the recombinant goose SAA and the production of specific anti-goose-SAA antisera (the full sequence of the goose SAA mRNA was also determined by applying 5′ and 3′ RACE-PCR). Using the antibody, highly specific and sensitive ELISA, immunohistochemical procedure and scintigraphy of the SAA has been introduced. The goose specific methods described herein may make an early detection of the disease possible in the near future.
|Translated title of the contribution||Molecular examination of goose amiloidosis. Author's summary|
|Number of pages||8|
|Journal||Magyar Allatorvosok Lapja|
|Publication status||Published - Oct 1 2004|
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