Molecular evolution of phosphoprotein phosphatases in Drosophila

Márton Miskei, Csaba Ádám, László Kovács, Z. Karányi, V. Dombrádi

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Phosphoprotein phosphatases (PPP), these ancient and important regulatory enzymes are present in all eukaryotic organisms. Based on the genome sequences of 12 Drosophila species we traced the evolution of the PPP catalytic subunits and noted a substantial expansion of the gene family. We concluded that the 18-22 PPP genes of Drosophilidae were generated from a core set of 8 indispensable phosphatases that are present in most of the insects. Retropositons followed by tandem gene duplications extended the phosphatase repertoire, and sporadic gene losses contributed to the species specific variations in the PPP complement. During the course of these studies we identified 5, up till now uncharacterized phosphatase retrogenes: PpY+, PpD5+, PpD6+, Pp4+, and Pp6+ which are found only in some ancient Drosophila. We demonstrated that all of these new PPP genes exhibit a distinct male specific expression. In addition to the changes in gene numbers, the intron-exon structure and the chromosomal localization of several PPP genes was also altered during evolution. The G-C content of the coding regions decreased when a gene moved into the heterochromatic region of chromosome Y. Thus the PPP enzymes exemplify the various types of dynamic rearrangements that accompany the molecular evolution of a gene family in Drosophilidae.

Original languageEnglish
Article numbere22218
JournalPLoS One
Volume6
Issue number7
DOIs
Publication statusPublished - 2011

Fingerprint

phosphoprotein phosphatase
Molecular Evolution
Phosphoprotein Phosphatases
Drosophila
Genes
Drosophilidae
genes
Phosphoric Monoester Hydrolases
Gene Duplication
Y Chromosome
Base Composition
Y chromosome
protein subunits
gene duplication
Enzymes
enzymes
Introns
exons
Insects
introns

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Molecular evolution of phosphoprotein phosphatases in Drosophila. / Miskei, Márton; Ádám, Csaba; Kovács, László; Karányi, Z.; Dombrádi, V.

In: PLoS One, Vol. 6, No. 7, e22218, 2011.

Research output: Contribution to journalArticle

Miskei, Márton ; Ádám, Csaba ; Kovács, László ; Karányi, Z. ; Dombrádi, V. / Molecular evolution of phosphoprotein phosphatases in Drosophila. In: PLoS One. 2011 ; Vol. 6, No. 7.
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