Molecular epidemiology of human calicivirus gastroenteritis outbreaks in Hungary, 1998 to 2000

G. Reuter, Tibor Farkas, Tamás Berke, Xi Jiang, David O. Matson, György Szücs

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Between November 1998 and November 2000, 196 stool specimens from 21 outbreaks of acute nonbacterial gastroenteritis occurring in 11 of the 19 counties of Hungary were collected and tested for human caliciviruses. Human caliciviruses were detected and characterized by a type-common enzyme-linked immunosorbent assay (EIA) and reverse transcription-polymerase chain reaction (RT-PCR) followed by cloning and sequencing. Twenty (95%) and 14 (67%) outbreaks were positive by EIA and RT-PCR, respectively, and 12 RT-PCR-positive outbreaks were also confirmed by sequencing. Comparative sequence analysis revealed 13 Norwalk-like virus sequences in the 12 outbreaks, including 11 Norwalk-like virus genogroup II (seven in Hawaii-like, two Lordsdale-like, one Melksham-like, and one Hillingdon-like) and two Norwalk-like virus genogroup I (related to Southampton-like and Desert Shield-like clusters) viruses. Multiple Norwalk-like virus clusters, with a predominance of Hawaii-like viruses, played an important role in nonbacterial gastroenteritis outbreaks during the study period. This is the first country-wide molecular epidemiological investigation of human calicivirus-associated, gastroenteritis outbreaks in Hungary and Central-Eastern Europe.

Original languageEnglish
Pages (from-to)390-398
Number of pages9
JournalJournal of Medical Virology
Volume68
Issue number3
DOIs
Publication statusPublished - Nov 1 2002

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Molecular Epidemiology
Hungary
Gastroenteritis
Disease Outbreaks
Norovirus
Reverse Transcription
Polymerase Chain Reaction
Enzyme-Linked Immunosorbent Assay
Genotype
Viruses
Eastern Europe
Sequence Analysis
Organism Cloning

Keywords

  • EIA
  • Norwalk-like viruses
  • RT-PCR
  • Sequencing

ASJC Scopus subject areas

  • Virology

Cite this

Molecular epidemiology of human calicivirus gastroenteritis outbreaks in Hungary, 1998 to 2000. / Reuter, G.; Farkas, Tibor; Berke, Tamás; Jiang, Xi; Matson, David O.; Szücs, György.

In: Journal of Medical Virology, Vol. 68, No. 3, 01.11.2002, p. 390-398.

Research output: Contribution to journalArticle

Reuter, G. ; Farkas, Tibor ; Berke, Tamás ; Jiang, Xi ; Matson, David O. ; Szücs, György. / Molecular epidemiology of human calicivirus gastroenteritis outbreaks in Hungary, 1998 to 2000. In: Journal of Medical Virology. 2002 ; Vol. 68, No. 3. pp. 390-398.
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