Molecular detection and genome analysis of circoviruses of European eel (Anguilla Anguilla) and sichel (Pelecus Cultratus)

Réka Borzák, Boglárka Sellyei, C. Székely, Andor Doszpoly

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The prevalence and distribution of piscine circoviruses (CVs) were tested in a routine virus monitoring programme in Lake Balaton, Hungary. A high prevalence of European eel CV (EeCV) was found in the apparently healthy eel population (35.5%). The copy number of the viral DNA in different organs was determined by quantitative real-time PCR. The results suggested that some eel specimens were in active viraemic status despite their asymptomatic condition. Furthermore, a novel, previously undescribed CV was also detected in eel and si-chel samples. Full genome characterisation confirmed that the virus represents a novel EeCV species (EeCV-2). The genome contains an integrated eel chromosome-derived fragment, suggesting that the original host of the virus was the eel and it probably emerged subsequently in the sichel by host switching. In some samples, an additional, 1,111-nt-long circular ssDNA was also observed involving a CV-like stem-loop structure and an ORF showing homology to CV capsid protein genes, without any sign of a replication initiator protein sequence.

Original languageEnglish
Pages (from-to)262-277
Number of pages16
JournalActa Veterinaria Hungarica
Volume65
Issue number2
DOIs
Publication statusPublished - Jun 1 2017

Fingerprint

Circoviridae
Circovirus
Anguilla
Eels
Anguilla anguilla
eel
Genome
genome
viruses
Viruses
single-stranded DNA
coat proteins
Hungary
open reading frames
quantitative polymerase chain reaction
Asymptomatic Diseases
amino acid sequences
Pelecus cultratus
Viral DNA
Capsid Proteins

Keywords

  • Circoviridae
  • Circovirus
  • European eel
  • Sichel

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Molecular detection and genome analysis of circoviruses of European eel (Anguilla Anguilla) and sichel (Pelecus Cultratus). / Borzák, Réka; Sellyei, Boglárka; Székely, C.; Doszpoly, Andor.

In: Acta Veterinaria Hungarica, Vol. 65, No. 2, 01.06.2017, p. 262-277.

Research output: Contribution to journalArticle

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