Molecular cytogenetic identification of a wheat-Aegilops geniculata Roth spontaneous chromosome substitution and its effects on the growth and physiological responses of seedlings to osmotic stress

Svetlana Landjeva, Konstantina Kocheva, Tania Karceva, Adél Sepsi, István Molnár, Annamária Schneider, Ganka Ganeva, Georgi Georgiev, M. Molnár-Láng

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The karyotypic constitution of a wheat-Aegilops geniculata 2M g(2A) disomic substitution line, isolated from backcross progenies of a wheat-Ae. geniculata amphiploid, was determined using multicolour fluorescence in situ hybridization and genomic in situ hybridization. The ability of the 2M g chromosome to compensate for wheat chromosome 2A was studied in relation to phenotypic traits and the growth and physiological responses of seedlings to polyethylene glycol (PEG)-induced osmotic stress. Plants of the substitution line had altered spike and seed morphologies, increased tillering, moderately reduced productivity and maintained better seedling growth in stress conditions. PEG treatment caused severe stress, resulting in considerable water loss from the leaves. Under this stress, the substitution line retained better leaf water status and suffered less oxidative damage than the wheat parent, as indicated by higher relative water content and lower malondialdehyde accumulation. These changes were consistent with more sustained membrane stability in the substitution line, evidenced by the lower value of the membrane injury index and the less-prominent increase in ion efflux from the apoplast.

Original languageEnglish
Pages (from-to)81-87
Number of pages7
JournalPlant Breeding
Volume131
Issue number1
DOIs
Publication statusPublished - Feb 2012

Fingerprint

Aegilops geniculata
chromosome substitution
substitution lines
Osmotic Pressure
osmotic stress
Seedlings
cytogenetics
Cytogenetics
Triticum
plant response
Chromosomes
wheat
seedlings
Growth
polyethylene glycol
In Situ Hybridization
Water
chromosomes
disomics
Membranes

Keywords

  • Drought
  • Goat-grass
  • Membrane stability
  • Seedling growth
  • Stress tolerance
  • Triticum aestivum

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science
  • Genetics

Cite this

Molecular cytogenetic identification of a wheat-Aegilops geniculata Roth spontaneous chromosome substitution and its effects on the growth and physiological responses of seedlings to osmotic stress. / Landjeva, Svetlana; Kocheva, Konstantina; Karceva, Tania; Sepsi, Adél; Molnár, István; Schneider, Annamária; Ganeva, Ganka; Georgiev, Georgi; Molnár-Láng, M.

In: Plant Breeding, Vol. 131, No. 1, 02.2012, p. 81-87.

Research output: Contribution to journalArticle

Landjeva, Svetlana ; Kocheva, Konstantina ; Karceva, Tania ; Sepsi, Adél ; Molnár, István ; Schneider, Annamária ; Ganeva, Ganka ; Georgiev, Georgi ; Molnár-Láng, M. / Molecular cytogenetic identification of a wheat-Aegilops geniculata Roth spontaneous chromosome substitution and its effects on the growth and physiological responses of seedlings to osmotic stress. In: Plant Breeding. 2012 ; Vol. 131, No. 1. pp. 81-87.
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