Molecular cytogenetic identification of a novel hexaploid wheat–thinopyrum intermedium partial amphiploid with high protein content

Mariyana Georgieva, Klaudia Kruppa, Nedyalka Tyankova, M. Molnár-Láng

Research output: Contribution to journalArticle

Abstract

Genomic in situ hybridization and fluorescence in situ hybridization were used to define the cytogenetic constitution of the intergeneric introgression line 29(1-57) obtained from the wheat × Thinopyrum intermedium (wheatgrass) cross. This line is a highprotein line resistant to leaf rust, yellow rust, and powdery mildew under field conditions and has 42 chromosomes. GISH analysis using a total genomic DNA probe from Thinopyrum intermedium resulted in the identification of 14 chromosomes of wheatgrass origin. The sequential GISH patterns obtained using St-genomic and J-genomic DNA as probes and ABD-genomic DNA from wheat as a blocker led to a better determination of the genomic type of wheatgrass chromosomes. Rearrangements involving Thinopyrum chromosomes and the A, B, and D genomes of wheat were not observed. FISH using the repetitive DNA probes pSc119.2, Afa-family, and pTa71 allowed for identification of all the wheat chromosomes present.

Original languageEnglish
Pages (from-to)554-560
Number of pages7
JournalTurkish Journal of Biology
Volume40
Issue number3
DOIs
Publication statusPublished - 2016

Fingerprint

hexaploidy
cytogenetics
Cytogenetics
Triticum
DNA Probes
protein content
genomics
Chromosomes
chromosomes
Thinopyrum intermedium
wheat
Proteins
Chromosomes, Human, 13-15
DNA probes
Chromosomes, Human, 4-5
Chromosomes, Human, 1-3
Chromosomes, Human, Pair 14
Constitution and Bylaws
Fluorescence In Situ Hybridization
Thinopyrum

Keywords

  • FISH
  • GISH
  • Wheat–Thinopyrum intermedium introgression line

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Physiology
  • Microbiology
  • Cell Biology
  • Agricultural and Biological Sciences(all)

Cite this

Molecular cytogenetic identification of a novel hexaploid wheat–thinopyrum intermedium partial amphiploid with high protein content. / Georgieva, Mariyana; Kruppa, Klaudia; Tyankova, Nedyalka; Molnár-Láng, M.

In: Turkish Journal of Biology, Vol. 40, No. 3, 2016, p. 554-560.

Research output: Contribution to journalArticle

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