Molecular cytogenetic (FISH) and genome analysis of diploid wheatgrasses and their phylogenetic relationship

G. Linc, Eszter Gaal, Istvan Molnar, Diana Icsoa, Ekaterina Badaeva, Maarta Molnaar-Lang

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

This paper reports detailed FISH-based karyotypes for three diploid wheatgrass species Agropyron cristatum (L.) Beauv., Thinopyrum bessarabicum (Savul.&Rayss) A. LoÈve, Pseudoroegneria spicata (Pursh) A. LoÈve, the supposed ancestors of hexaploid Thinopyrum intermedium (Host) Barkworth & D.R.Dewey, compiled using DNA repeats and comparative genome analysis based on COS markers. Fluorescence in situ hybridization (FISH) with repetitive DNA probes proved suitable for the identification of individual chromosomes in the diploid JJ, StSt and PP genomes. Of the seven microsatellite markers tested only the (GAA)n trinucleotide sequence was appropriate for use as a single chromosome marker for the P. spicata AS chromosome. Based on COS marker analysis, the phylogenetic relationship between diploid wheatgrasses and the hexaploid bread wheat genomes was established. These findings confirmed that the J and E genomes are in neighbouring clusters.

Original languageEnglish
Article numbere0173623
JournalPLoS One
Volume12
Issue number3
DOIs
Publication statusPublished - Mar 1 2017

Fingerprint

fluorescence in situ hybridization
Diploidy
Fluorescence In Situ Hybridization
cytogenetics
Cytogenetics
diploidy
Chromosomes
Genes
Fluorescence
Genome
Pseudoroegneria spicata
genome
Love
phylogeny
hexaploidy
chromosomes
Thinopyrum bessarabicum
Agropyron
Thinopyrum intermedium
Agropyron cristatum

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Molecular cytogenetic (FISH) and genome analysis of diploid wheatgrasses and their phylogenetic relationship. / Linc, G.; Gaal, Eszter; Molnar, Istvan; Icsoa, Diana; Badaeva, Ekaterina; Molnaar-Lang, Maarta.

In: PLoS One, Vol. 12, No. 3, e0173623, 01.03.2017.

Research output: Contribution to journalArticle

Linc, G. ; Gaal, Eszter ; Molnar, Istvan ; Icsoa, Diana ; Badaeva, Ekaterina ; Molnaar-Lang, Maarta. / Molecular cytogenetic (FISH) and genome analysis of diploid wheatgrasses and their phylogenetic relationship. In: PLoS One. 2017 ; Vol. 12, No. 3.
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