Molecular cytogenetic characterization of Aegilops biuncialis and its use for the identification of 5 derived wheat - Aegilops biuncialis disomic addition lines

Annamária Schneider, Gabriella Linc, István Molnár, Márta Molnár-Láng

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

The aim of the experiments was to produce and identify different Triticum aestivum - Aegilops biuncialis disomic addition lines. To facilitate the exact identification of the Ae. biuncialis chromosomes in these Triticum aestivum - Ae. biuncialis disomic additions, it was necessary to analyze the fluorescence in situ hybridization (FISH) pattern of Ae. biuncialis (2n = 4x = 28, U bUbMbMb), comparing it with the diploid progenitors (Aegilops umbellulata, 2n = 2x = 14, UU and Aegilops comosa, 2n = 2x = 14, MM). To identify the Ae. biuncialis chromosomes, FISH was carried out using 2 DNA clones (pSc119.2 and pAs1) on Ae. biuncialis and its 2 diploid progenitor species. Differences in the hybridization patterns of all chromosomes were observed among the 4 Ae. umbellulata accessions, the 4 Ae. comosa accessions, and the 3 Ae. biuncialis accessions analyzed. The hybridization pattern of the M genome was more variable than that of the U genome. Five different wheat - Ae. biuncialis addition lines were produced from the wheat - Ae. biuncialis amphiploids produced earlier in Martonvásár. The 2M, 3M, 7M, 3U, and 5U chromosome pairs were identified with FISH using 3 repetitive DNA clones (pSc119.2, pAs1, and pTa71) in the disomic chromosome additions produced. Genomic in situ hybridization (GISH) was used to differentiate the Ae. biuncialis chromosomes from wheat, but no chromosome rearrangements between wheat and Ae. biuncialis were detected in the addition lines.

Original languageEnglish
Pages (from-to)1070-1082
Number of pages13
JournalGenome
Volume48
Issue number6
DOIs
Publication statusPublished - Dec 2005

Keywords

  • Aegilops biuncialis
  • Fluorescence in situ hybridization
  • Genomic in situ hybridization
  • Triticum aestivum
  • Wheat - Aegilops biuncialis addition lines

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'Molecular cytogenetic characterization of Aegilops biuncialis and its use for the identification of 5 derived wheat - Aegilops biuncialis disomic addition lines'. Together they form a unique fingerprint.

  • Cite this