Molecular cytogenetic characterization of a novel cell line established from a superficial spreading melanoma

Andrea Treszl, Andrea Ladanyi, Zsuzsa Rakosy, Zsuzsa Buczko, Roza Adany, Margit Balazs

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9 Citations (Scopus)

Abstract

We report the complex cytogenetic analysis of a novel melanoma cell line (M35/01) established from a vertical growth phase of a superficial spreading melanoma. Similarly to its parental tumor, this cell line metastasizes to the liver. Using combined molecular cytogenetic techniques, we could identify a reservoir of chromosomal alterations in M35/01. In addition, we had sufficient amount of DNA from both the original primary tumor and the cell line which allowed for the comparison of their genetic patterns by chromosomal CGH. Several common alterations were found indicating the same clonal origin. These alterations included gains of 6p, 7q, 15q and deletions of 9, 10, 16q and 17p. Chromosomal losses present only in the cell line were detected on chromosome 4,16p, 18 and gains on 20pl2-qter. Array CGH analysis of the M35/01 cell line provided similar results with a much higher resolution, representing relatively high level gains on 7q31.2-q31.31, 15q25, 20q, and losses on 4q28, 9p21-p24, 9q21-q22, 10q25, 16q13-q23, 17p12-13 and 18q12-23. Using SKY-FISH, several structural alterations could be detected which were not recognized by conventional cytogenetics. Except for chromosome 18, none of the centromeres showed normal distribution by FISH. Our analysis shows that a high number of chromosomal alterations, which are known to be nonrandomly associated with melanoma progression, can be found by the combined use of different molecular genetic techniques. This new melanoma cell line would be an excellent model for investigating the mechanism of organ specific-metastatic events of malignant melanoma.

Original languageEnglish
Pages (from-to)1844-1853
Number of pages10
JournalFrontiers in Bioscience
Volume11
Issue number2 P.1591-2006
Publication statusPublished - Feb 24 2006

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Keywords

  • Array CGH
  • Comparative Genomic Hybridization
  • Fluorescence In Situ Hybridization
  • Melanoma cell line
  • Spectral Karyotyping

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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