Molecular cytogenetic characterization and SSR marker analysis of a leaf rust resistant wheat line carrying a 6G(6B) substitution from Triticum timopheevii (Zhuk.)

Andrea Uhrin, Éva Szakács, L. Láng, Z. Bedő, M. Molnár-Láng

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A disease (powdery mildew, leaf rust) resistant line was selected from the progenies of a Triticum aestivum × Triticum timopheevii amphiploid produced at Martonvásár. This line was previously identified with C-banding as a 6G(6B) substitution. In order to detect the 6G chromosome in a wheat background, fluorescence in situ hybridization (FISH) and microsatellite marker analysis were used. Ten microsatellite markers of the 43 tested generated PCR products that were polymorphic between chromosomes 6B and 6G, and four showed length-polymorphism. The FISH hybridization pattern of 6G from T. timopheevii was identified using a combination of four repetitive DNA probes (Afa-family, pSc119. 2, pTa71, (GAA) 7). Genomic in situ hybridization (GISH) technique, capable of labelling the A t and G genomes separately, was used on the same slides to differentiate the A t and G genomes in T. timopheevii. The A t and G genomes of T. timopheevii were grouped on the basis of the GISH patterns and a cyclic intergenomic translocation involving 6A t-1G-4G was detected in T. timopheevii accession TRI667. The presence of 6G in the substitution line was demonstrated using FISH with the four repetitive DNA probes. Chromosome 6G was clearly identified and its FISH pattern was different from that of 6B in the parental wheat cultivar Fleischmann-481. According to field tests, the 6G(6B) substitution line has resistance to leaf rust.

Original languageEnglish
Pages (from-to)45-55
Number of pages11
JournalEuphytica
Volume186
Issue number1
DOIs
Publication statusPublished - Jul 2012

Fingerprint

Triticum timopheevii
leaf rust
Fluorescence In Situ Hybridization
cytogenetics
Cytogenetics
Triticum
fluorescence in situ hybridization
wheat
Chromosomes
DNA Probes
Genome
substitution lines
Microsatellite Repeats
DNA probes
In Situ Hybridization
chromosomes
in situ hybridization
genome
microsatellite repeats
genomics

Keywords

  • Fluorescence in situ hybridization
  • Leaf rust resistance
  • Microsatellite marker
  • T. timopheevii
  • Triticum aestivum

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Horticulture
  • Plant Science

Cite this

@article{af2308a79663483baee8cc11f7c5d0b0,
title = "Molecular cytogenetic characterization and SSR marker analysis of a leaf rust resistant wheat line carrying a 6G(6B) substitution from Triticum timopheevii (Zhuk.)",
abstract = "A disease (powdery mildew, leaf rust) resistant line was selected from the progenies of a Triticum aestivum × Triticum timopheevii amphiploid produced at Martonv{\'a}s{\'a}r. This line was previously identified with C-banding as a 6G(6B) substitution. In order to detect the 6G chromosome in a wheat background, fluorescence in situ hybridization (FISH) and microsatellite marker analysis were used. Ten microsatellite markers of the 43 tested generated PCR products that were polymorphic between chromosomes 6B and 6G, and four showed length-polymorphism. The FISH hybridization pattern of 6G from T. timopheevii was identified using a combination of four repetitive DNA probes (Afa-family, pSc119. 2, pTa71, (GAA) 7). Genomic in situ hybridization (GISH) technique, capable of labelling the A t and G genomes separately, was used on the same slides to differentiate the A t and G genomes in T. timopheevii. The A t and G genomes of T. timopheevii were grouped on the basis of the GISH patterns and a cyclic intergenomic translocation involving 6A t-1G-4G was detected in T. timopheevii accession TRI667. The presence of 6G in the substitution line was demonstrated using FISH with the four repetitive DNA probes. Chromosome 6G was clearly identified and its FISH pattern was different from that of 6B in the parental wheat cultivar Fleischmann-481. According to field tests, the 6G(6B) substitution line has resistance to leaf rust.",
keywords = "Fluorescence in situ hybridization, Leaf rust resistance, Microsatellite marker, T. timopheevii, Triticum aestivum",
author = "Andrea Uhrin and {\'E}va Szak{\'a}cs and L. L{\'a}ng and Z. Bedő and M. Moln{\'a}r-L{\'a}ng",
year = "2012",
month = "7",
doi = "10.1007/s10681-011-0483-1",
language = "English",
volume = "186",
pages = "45--55",
journal = "Euphytica",
issn = "0014-2336",
publisher = "Springer Netherlands",
number = "1",

}

TY - JOUR

T1 - Molecular cytogenetic characterization and SSR marker analysis of a leaf rust resistant wheat line carrying a 6G(6B) substitution from Triticum timopheevii (Zhuk.)

AU - Uhrin, Andrea

AU - Szakács, Éva

AU - Láng, L.

AU - Bedő, Z.

AU - Molnár-Láng, M.

PY - 2012/7

Y1 - 2012/7

N2 - A disease (powdery mildew, leaf rust) resistant line was selected from the progenies of a Triticum aestivum × Triticum timopheevii amphiploid produced at Martonvásár. This line was previously identified with C-banding as a 6G(6B) substitution. In order to detect the 6G chromosome in a wheat background, fluorescence in situ hybridization (FISH) and microsatellite marker analysis were used. Ten microsatellite markers of the 43 tested generated PCR products that were polymorphic between chromosomes 6B and 6G, and four showed length-polymorphism. The FISH hybridization pattern of 6G from T. timopheevii was identified using a combination of four repetitive DNA probes (Afa-family, pSc119. 2, pTa71, (GAA) 7). Genomic in situ hybridization (GISH) technique, capable of labelling the A t and G genomes separately, was used on the same slides to differentiate the A t and G genomes in T. timopheevii. The A t and G genomes of T. timopheevii were grouped on the basis of the GISH patterns and a cyclic intergenomic translocation involving 6A t-1G-4G was detected in T. timopheevii accession TRI667. The presence of 6G in the substitution line was demonstrated using FISH with the four repetitive DNA probes. Chromosome 6G was clearly identified and its FISH pattern was different from that of 6B in the parental wheat cultivar Fleischmann-481. According to field tests, the 6G(6B) substitution line has resistance to leaf rust.

AB - A disease (powdery mildew, leaf rust) resistant line was selected from the progenies of a Triticum aestivum × Triticum timopheevii amphiploid produced at Martonvásár. This line was previously identified with C-banding as a 6G(6B) substitution. In order to detect the 6G chromosome in a wheat background, fluorescence in situ hybridization (FISH) and microsatellite marker analysis were used. Ten microsatellite markers of the 43 tested generated PCR products that were polymorphic between chromosomes 6B and 6G, and four showed length-polymorphism. The FISH hybridization pattern of 6G from T. timopheevii was identified using a combination of four repetitive DNA probes (Afa-family, pSc119. 2, pTa71, (GAA) 7). Genomic in situ hybridization (GISH) technique, capable of labelling the A t and G genomes separately, was used on the same slides to differentiate the A t and G genomes in T. timopheevii. The A t and G genomes of T. timopheevii were grouped on the basis of the GISH patterns and a cyclic intergenomic translocation involving 6A t-1G-4G was detected in T. timopheevii accession TRI667. The presence of 6G in the substitution line was demonstrated using FISH with the four repetitive DNA probes. Chromosome 6G was clearly identified and its FISH pattern was different from that of 6B in the parental wheat cultivar Fleischmann-481. According to field tests, the 6G(6B) substitution line has resistance to leaf rust.

KW - Fluorescence in situ hybridization

KW - Leaf rust resistance

KW - Microsatellite marker

KW - T. timopheevii

KW - Triticum aestivum

UR - http://www.scopus.com/inward/record.url?scp=84861478643&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84861478643&partnerID=8YFLogxK

U2 - 10.1007/s10681-011-0483-1

DO - 10.1007/s10681-011-0483-1

M3 - Article

AN - SCOPUS:84861478643

VL - 186

SP - 45

EP - 55

JO - Euphytica

JF - Euphytica

SN - 0014-2336

IS - 1

ER -