Molecular cytogenetic characterisation of Salix viminalis L. using repetitive DNA sequences

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Salix viminalis L. (2n = 38) is a diploid dicot species belonging to the Salix genus of the Salicaceae family. This short-rotation woody crop is one of the most important renewable bioenergy resources worldwide. In breeding for high biomass productivity, limited knowledge is available on the molecular cytogenetics of willow, which could be combined with genetic linkage mapping. The present paper describes the adaptation of a fluorescence in situ hybridisation (FISH) protocol as a new approach to analyse the genomic constitution of Salix viminalis using the heterologous DNA clones pSc119.2, pTa71, pTa794, pAs1, Afa-family, pAl1, HT100.3, ZCF1 and the GAA microsatellite marker. Three of the nine probes showed unambiguous signals on the metaphase chromosomes. FISH analysis with the pTa71 probe detected one major 18S-5.8S-26S rDNA locus on the short arm of one chromosome pair; however, the pTa794 rDNA site was not visible. One chromosome pair showed a distinct signal around the centromeric region after FISH with the telomere-specific DNA clone HT100.3. Two chromosome pairs were found to have pAs1 FISH signals, which represent a D-genome-specific insert from Aegilops tauschii. Based on the FISH study, a set of chromosomes with characteristic patterns is presented, which could be used to establish the karyotype of willow species.

Original languageEnglish
Pages (from-to)265-269
Number of pages5
JournalJournal of Applied Genetics
Volume54
Issue number3
DOIs
Publication statusPublished - Aug 2 2013

Keywords

  • FISH
  • Genome constitution
  • Repetitive DNA clones
  • Salix viminalis

ASJC Scopus subject areas

  • Genetics

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