Molecular cytogenetic analysis of triticum aestivum and Thinopyrum intermedium using the fish technique

Mariyana Georgieva, Adel Sepsi, Marta Molnar-Lang, Nedyalka Tyankova

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Fluorescence in situ hybridization (FISH), using repetitive DNA probes from Aegilops squarrosa L. (Afa family), Secale cereale (pSc119.2) and barley containing the ribosomal genes 18S-5.8S-26S (pTA71), was used to characterize the chromosome constitution of the two Triticum aestivum (2n = 6x = 42) cultivars Sadovo and Russalka and the segmental autoallohexaploid species Thinopyrum intermedium (2n = 6x = 42). FISH results indicated that all repetitive probes made clear signals on wheat and on most of the wheatgrass chromosomes. The genomic constitution of the wheat cultivars comprised ABD genomes and no differences were found between their chromosomes with respect to repeated sequences. Four Th. intermedium chromosomal pairs hybridized with pSc119.2. Two of these were shown to possess polymorphic pericentromeric sites. FISH with Afa family detected polymorphic sites on 11 chromosomal wheatgrass pairs. The pTa71 probe displayed a signal on 4 pairs. Three pairs were not appropriate because they did not hybridize with any of the probes used.

Original languageEnglish
Pages (from-to)1713-1718
Number of pages6
JournalComptes Rendus de L'Academie Bulgare des Sciences
Volume64
Issue number12
Publication statusPublished - Dec 1 2011

Keywords

  • FISH
  • Thinopyrum intermedium
  • Triticum aestivum

ASJC Scopus subject areas

  • General

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