Molecular cytogenetic analysis of the wheat-Agropyron elongatum partial amphiploid BE-1

Adél Sepsi, István Molnár, Dezso Szalay, Márta Molnár-Láng

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Multicolor genomic in situ hybridization (mcGISH) and fluorescence in situ hybridization (FISH) using repetitive DNA probes were used to characterize the genomic composition of the wheat-Thinopyrum ponticum partial amphiploid BE-1. The amphiploid is a high-protein line having resistance to leaf rust (Puccinia recondita f. sp. tritici) and powdery mildew (Blumeria graminis f. sp. tritici) and has in total 56 chromosomes per cell. Multicolor GISH revealed 16 chromosomes originating from Thinopyrum ponticum and 14 A genome, 14 B genome and 12 D genome chromosomes. Rearrangements involving Thinopyrum chromosomes and the A, B and D genomes of wheat were visualised. FISH using repetitive DNA probes allowed the identification of all wheat chromosomes present and the determination of the chromosomes involved in translocations.

Original languageEnglish
Pages (from-to)139-141
Number of pages3
JournalActa Biologica Szegediensis
Volume52
Issue number1
Publication statusPublished - Dec 1 2008

Keywords

  • Amphiploid
  • FISH
  • Multicolor GISH
  • Thinopyrum ponticum
  • Triticum aestivum

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

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