Molecular cytogenetic analysis of Aegilops cylindrica host

G. Linc, Bernd R. Friebe, Ralf G. Kynast, M. Molnár-Láng, Bela Köszegi, J. Sutka, Bikram S. Gill

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

The genomic constitution of Aegilops cylindrica Host (2n = 4x = 28, D(c)D(c)C(c)C(c)) was analyzed by C-banding, genomic in situ hybridization (GISH), and fluorescence in situ hybridization (FISH) using the DNA clones pSc119, pAs1, pTa71, and pTA794. The C-banding patterns of the D(c)- and C(c)-genome chromosomes of Ae. cylindrica are similar to those of D-and C-genome chromosomes of the diploid progenitor species Ae. tauschii Coss. and Ae. caudata L., respectively. These similarities permitted the genome allocation and identification of the homoeologous relationships of the Ae. cylindrica chromosomes. FISH analysis detected one major 18S-5.8S-25S rDNA locus in the short arm of chromosome lC(c). Minor 18S-5.8S-25S rDNA loci were mapped in the short arms of 5D(c) and 5C(c). 5S rDNA loci were identified in the short arm of chromosomes lC(c). 5D(c), 5C(c), and 1D(c). GISH analysis detected intergenomic translocation in three of the five Ae. cylindrica accessions. The breakpoints in all translocations were non-centromeric with similar-sized segment exchanges.

Original languageEnglish
Pages (from-to)497-503
Number of pages7
JournalGenome
Volume42
Issue number3
Publication statusPublished - 1999

Fingerprint

Aegilops cylindrica
cytogenetic analysis
Cytogenetic Analysis
Dilatation and Curettage
Chromosomes
chromosomes
Ribosomal DNA
chromosome banding
Genome
fluorescence in situ hybridization
Fluorescence In Situ Hybridization
genomics
in situ hybridization
loci
In Situ Hybridization
genome
Urodela
Caudata
Constitution and Bylaws
Diploidy

Keywords

  • Aegilops cylindrica
  • C-banding
  • FISH
  • Genome evolution
  • GISH

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biotechnology
  • Genetics
  • Genetics(clinical)

Cite this

Linc, G., Friebe, B. R., Kynast, R. G., Molnár-Láng, M., Köszegi, B., Sutka, J., & Gill, B. S. (1999). Molecular cytogenetic analysis of Aegilops cylindrica host. Genome, 42(3), 497-503.

Molecular cytogenetic analysis of Aegilops cylindrica host. / Linc, G.; Friebe, Bernd R.; Kynast, Ralf G.; Molnár-Láng, M.; Köszegi, Bela; Sutka, J.; Gill, Bikram S.

In: Genome, Vol. 42, No. 3, 1999, p. 497-503.

Research output: Contribution to journalArticle

Linc, G, Friebe, BR, Kynast, RG, Molnár-Láng, M, Köszegi, B, Sutka, J & Gill, BS 1999, 'Molecular cytogenetic analysis of Aegilops cylindrica host', Genome, vol. 42, no. 3, pp. 497-503.
Linc G, Friebe BR, Kynast RG, Molnár-Láng M, Köszegi B, Sutka J et al. Molecular cytogenetic analysis of Aegilops cylindrica host. Genome. 1999;42(3):497-503.
Linc, G. ; Friebe, Bernd R. ; Kynast, Ralf G. ; Molnár-Láng, M. ; Köszegi, Bela ; Sutka, J. ; Gill, Bikram S. / Molecular cytogenetic analysis of Aegilops cylindrica host. In: Genome. 1999 ; Vol. 42, No. 3. pp. 497-503.
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