Molecular cytogenetic analysis of Aegilops cylindrica host

Gabriella Linc, Bernd R. Friebe, Ralf G. Kynast, Marta Molnar-Lang, Bela Köszegi, Jozsef Sutka, Bikram S. Gill

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Abstract

The genomic constitution of Aegilops cylindrica Host (2n = 4x = 28, D(c)D(c)C(c)C(c)) was analyzed by C-banding, genomic in situ hybridization (GISH), and fluorescence in situ hybridization (FISH) using the DNA clones pSc119, pAs1, pTa71, and pTA794. The C-banding patterns of the D(c)- and C(c)-genome chromosomes of Ae. cylindrica are similar to those of D-and C-genome chromosomes of the diploid progenitor species Ae. tauschii Coss. and Ae. caudata L., respectively. These similarities permitted the genome allocation and identification of the homoeologous relationships of the Ae. cylindrica chromosomes. FISH analysis detected one major 18S-5.8S-25S rDNA locus in the short arm of chromosome lC(c). Minor 18S-5.8S-25S rDNA loci were mapped in the short arms of 5D(c) and 5C(c). 5S rDNA loci were identified in the short arm of chromosomes lC(c). 5D(c), 5C(c), and 1D(c). GISH analysis detected intergenomic translocation in three of the five Ae. cylindrica accessions. The breakpoints in all translocations were non-centromeric with similar-sized segment exchanges.

Original languageEnglish
Pages (from-to)497-503
Number of pages7
JournalGenome
Volume42
Issue number3
Publication statusPublished - Jan 1 1999

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Keywords

  • Aegilops cylindrica
  • C-banding
  • FISH
  • GISH
  • Genome evolution

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Genetics

Cite this

Linc, G., Friebe, B. R., Kynast, R. G., Molnar-Lang, M., Köszegi, B., Sutka, J., & Gill, B. S. (1999). Molecular cytogenetic analysis of Aegilops cylindrica host. Genome, 42(3), 497-503.