Molecular comparison of the fibre proteins of bovine adenovirus subtype A and subtype B

Andrea Szendroi, Anita Schamberger, Tamás Bakonyi, Ákos Hornyák, Miklós Rusvai

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The fibre gene of the bovine adenovirus type 2 (BAdV2) subtype B was prepared for sequencing by using cloning, sub-cloning and PCR amplification techniques. The nucleotide sequence of the total fibre gene was determined, and it was found to consist of 1647 nucleotides, coding for a polypeptide of 549 amino acids. The fibre gene regions of BAdV2 A and B subtypes were aligned. The nucleotide identity of the total fibre gene was found to be 60.5%; however, the homology showed great differences in the different subregions coding for the shaft and knob part of the fibre, and the two subtypes were almost identical in the tail subregion. Remarkable changes indicating deletion, insertion and point mutations were found in the shaft subregion when BAdV2/A and B subtypes were compared. We concluded that the differences found in the haemagglutinating activity of the two subtypes of BAdV2 can mostly be explained by the changes in the polypeptide structure of the fibre shaft.

Original languageEnglish
Pages (from-to)59-63
Number of pages5
JournalVirus Research
Volume94
Issue number1
DOIs
Publication statusPublished - Jul 1 2003

Keywords

  • Bovine adenovirus type 2
  • DNA sequencing
  • Fibre protein
  • Sequence analyses

ASJC Scopus subject areas

  • Cancer Research
  • Virology
  • Infectious Diseases

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