Molecular cloning and chromosomal localization of a novel Drosophila protein phosphatase

V. Dombrádi, J. Myles Axton, David M. Glover, Patricia T W Cohen

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

A 1.0 kilobase cDNA coding for the complete amino acid sequence of a putative protein phosphatase (314 amino acid residues, molecular mass 36 kDa) has been isolated from a Drosophila head cDNA library. The cDNA hybridises to a single site on the right arm of the second chromosome at cytological position 55A1-3. The deduced sequence of the protein, designated protein phosphatase-Y, is homologous to the catalytic subunits of Drosophila and rabbit protein phosphatase- 1α (64 and 59% identity, respectively) and rabbit protein phosphatase-2A (39% identity). These and other comparisons demonstrate that this novel enzyme is not the Drosophila counterpart of mammalian protein phosphatases 1, 2A, 2B, 2C or X.

Original languageEnglish
Pages (from-to)391-395
Number of pages5
JournalFEBS Letters
Volume247
Issue number2
DOIs
Publication statusPublished - Apr 24 1989

Fingerprint

Drosophila Proteins
Protein Phosphatase 1
Protein Phosphatase 2
Cloning
Phosphoprotein Phosphatases
Molecular Cloning
Drosophila
Complementary DNA
Rabbits
Amino Acids
Molecular mass
Chromosomes
Gene Library
Amino Acid Sequence
Catalytic Domain
Arm
Head
Enzymes
Proteins
Drosophila And protein

Keywords

  • (Drosophila melanogaster)
  • Amino acid sequence
  • cDNA cloning
  • Nucleotide sequence
  • Protein phosphatase

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Molecular cloning and chromosomal localization of a novel Drosophila protein phosphatase. / Dombrádi, V.; Axton, J. Myles; Glover, David M.; Cohen, Patricia T W.

In: FEBS Letters, Vol. 247, No. 2, 24.04.1989, p. 391-395.

Research output: Contribution to journalArticle

Dombrádi, V. ; Axton, J. Myles ; Glover, David M. ; Cohen, Patricia T W. / Molecular cloning and chromosomal localization of a novel Drosophila protein phosphatase. In: FEBS Letters. 1989 ; Vol. 247, No. 2. pp. 391-395.
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